XT-5 PCR system , (3 U/µl)
₹13,167.00 + ₹2,370.06 GST
₹15,537.06 (Incl. of all taxes)
Description XT-5 PCR System is useful to amplify upto 5 kb target DNA from genomic DNA templates. A well-defined ratio of enzymes along with a set of highly optimized buffer systems (Assay buffers 5A and 5B) ensures specific PCR products. Assay buffer 5A is used for obtaining highly specific target DNA with suboptimal yield while assay buffer 5B is best utilized for higher yields.
XT-Polymerase buffer 5A : (1X) TAPS (pH 8.8), KCl, 1.75mM MgCl₂ and Gelatin. XT-Polymerase buffer 5B : (1X) Tris (pH 9.1), (NH₄) SO₄, 1.5mM MgCl₂. 2 Buffers supplied at 10X concentration.
Specification : Enzyme is supplied at a concentration of 3U/μl. With every order of XT-5 PCR system we supply: a. Enzyme: 1 vial b. 10X assay buffer 5A: 1 vial c. 10X assay buffer 5B: 1 vial
Application : • Useful for high efficiency PCR amplifications. The products obtained can be used for gene cloning and other genetic manipulations. • Ensures higher yields of the amplified product with improved fidelity.
Note: Concentration of the components of the buffer are proprietary.
Performance Test : XT-5 PCR system is tested for PCR amplifications of fragment sizes – 1.5 kb to 8.0 kb using both human genomic DNA and lambda DNA as templates. Sequence specific primers were used for amplifications.
Storage : -20°C
| Product Code | 0601800051730-MME18J |
|---|---|
| HSN Code | 35079099 |
| Brand | GeNei |
| Usually dispatched in | 2-3 WEEKS |
| Pack Size | 1000 U |
Description XT-5 PCR System is useful to amplify upto 5 kb target DNA from genomic DNA templates. A well-defined ratio of enzymes along with a set of highly optimized buffer systems (Assay buffers 5A and 5B) ensures specific PCR products. Assay buffer 5A is used for obtaining highly specific target DNA with suboptimal yield while assay buffer 5B is best utilized for higher yields.
XT-Polymerase buffer 5A : (1X) TAPS (pH 8.8), KCl, 1.75mM MgCl₂ and Gelatin. XT-Polymerase buffer 5B : (1X) Tris (pH 9.1), (NH₄) SO₄, 1.5mM MgCl₂. 2 Buffers supplied at 10X concentration.
Specification : Enzyme is supplied at a concentration of 3U/μl. With every order of XT-5 PCR system we supply: a. Enzyme: 1 vial b. 10X assay buffer 5A: 1 vial c. 10X assay buffer 5B: 1 vial
Application : • Useful for high efficiency PCR amplifications. The products obtained can be used for gene cloning and other genetic manipulations. • Ensures higher yields of the amplified product with improved fidelity.
Note: Concentration of the components of the buffer are proprietary.
Performance Test : XT-5 PCR system is tested for PCR amplifications of fragment sizes – 1.5 kb to 8.0 kb using both human genomic DNA and lambda DNA as templates. Sequence specific primers were used for amplifications.
Storage : -20°C