Western Blot Development Kit for Rabbit Antibody, 10 blots

DESCRIPTION:

Western Blot Development Kit (For Rabbit antibodies) utilizes
direct detection technique to detect rabbit antibody. The
antigen-immobilized membrane is incubated with its specific
antibody which is of Rabbit origin. The antibody is allowed to
bind to a biotinylated secondary antibody (anti Rabbit IgGbiotin
conjugate).Biotinylated secondary antibody increases
the sensitivity of the reaction . Biotin has strong affinity for
Streptavidin, which is labeled with Alkaline Phosphatase
(ALP). The membrane is then incubated with Streptavidin ALP
conjugate that binds to biotin on the antibody. This in turn is
detected by BCIP/NBT, substrate specific for alkaline
phosphatase. The development of blue-grey color indicates a
positive reaction and hence the presence of antigen on the
membrane and the antibody which has bound to it.

MATERIALS PROVIDED:
Blocking Buffer
Secondary Antibody – Biotin
Streptavidin – ALP
Wash Buffer
Assay Buffer
BCIP/NBT
Control Strips
Instruction Manual