Western Blot Development Kit for Human Antibody, 10 blots
₹12,096.00 + ₹1,451.52 GST
₹13,547.52 (Incl. of all taxes)
DESCRIPTION:
Western Blot Development Kit (For Human antibodies) utilizes direct detection technique to detect Human antibody. The antigen-immobilized membrane is incubated with its specific antibody which is of Human origin. The antibody is allowed to bind to a biotinylated secondary antibody (anti Human IgG-biotin conjugate).Biotinylated secondary antibody increases the sensitivity of the reaction . Biotin has strong affinity for Streptavidin, which is labeled with Alkaline Phosphatase (ALP). The membrane is then incubated with Streptavidin ALP conjugate that binds to biotin on the antibody. This in turn is detected by BCIP/NBT, substrate specific for alkaline phosphatase. The development of blue-grey color indicates a positive reaction and hence the presence of antigen on the membrane and the antibody which has bound to it.
MATERIALS PROVIDED:
Blocking Buffer
Secondary Antibody – Biotin
Streptavidin – ALP
Wash Buffer
Assay Buffer
BCIP/NBT
Control Strips
Instruction Manual
| Product Code | 2601300011730-KT13_ |
|---|---|
| HSN Code | 38220090 |
| Brand | GeNei |
| Usually dispatched in | 2-3 WEEKS |
| Pack Size | 1 EA |
DESCRIPTION:
Western Blot Development Kit (For Human antibodies) utilizes direct detection technique to detect Human antibody. The antigen-immobilized membrane is incubated with its specific antibody which is of Human origin. The antibody is allowed to bind to a biotinylated secondary antibody (anti Human IgG-biotin conjugate).Biotinylated secondary antibody increases the sensitivity of the reaction . Biotin has strong affinity for Streptavidin, which is labeled with Alkaline Phosphatase (ALP). The membrane is then incubated with Streptavidin ALP conjugate that binds to biotin on the antibody. This in turn is detected by BCIP/NBT, substrate specific for alkaline phosphatase. The development of blue-grey color indicates a positive reaction and hence the presence of antigen on the membrane and the antibody which has bound to it.
MATERIALS PROVIDED:
Blocking Buffer
Secondary Antibody – Biotin
Streptavidin – ALP
Wash Buffer
Assay Buffer
BCIP/NBT
Control Strips
Instruction Manual