Trypsin ex. Bovine Pancreas for cell culture, 0.2Anson U/g (2000U/g), Endotoxin (BET) 0.05EU/mg

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Overview

DESCRIPTION
Storage : -20 °C (Blue/Dry Ice)
Shelf Life : 60 Months

Specifications
Appearance (Colour) White
Appearance (Form) Crystalline powder
Solubility (Turbidity) 1% aq. solution Clear
Solubility (Colour) 1% aq. solution Colourless
Activity min 0.2 Anson units/g
min 50 BAEE units/mg
Suitability for Cell Culture Passes
Bacterial Endotoxin Test (BET) (EU/mg = IU/mg) <0.05 EU/mg
Unit Definition One BAEE unit of Trypsin activity will produce ΔA at 253 nm of 0.001 per minute with BAEE as substrate at pH 8.0 at 25 °C in a reaction volume of 3.0 mL.
3 BAEE units = 1 NF unit
Enzyme solution for activity determination Prepared in 0.001 N Tris HCl.
Notes
  1 Anson unit is amount of enzyme which under standard conditions of Anson hydrolyzes Haemoglobin at such an initial rate the amount of hydrolysis product formed per min has the same extinction on reaction with phenol reagent as 1 mmole of tyrosine Ref M.L.Anson J.gen Physiol 22,79,1939 & 17,151,1933
More Information
Product Code 52682-1 Kg
HSN Code 35079099
Brand SRL
Usually dispatched in 1 WEEK
Pack Size 1 KG
CAS Number 9002-07-7

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DESCRIPTION
Storage : -20 °C (Blue/Dry Ice)
Shelf Life : 60 Months

Specifications
Appearance (Colour) White
Appearance (Form) Crystalline powder
Solubility (Turbidity) 1% aq. solution Clear
Solubility (Colour) 1% aq. solution Colourless
Activity min 0.2 Anson units/g
min 50 BAEE units/mg
Suitability for Cell Culture Passes
Bacterial Endotoxin Test (BET) (EU/mg = IU/mg) <0.05 EU/mg
Unit Definition One BAEE unit of Trypsin activity will produce ΔA at 253 nm of 0.001 per minute with BAEE as substrate at pH 8.0 at 25 °C in a reaction volume of 3.0 mL.
3 BAEE units = 1 NF unit
Enzyme solution for activity determination Prepared in 0.001 N Tris HCl.
Notes
  1 Anson unit is amount of enzyme which under standard conditions of Anson hydrolyzes Haemoglobin at such an initial rate the amount of hydrolysis product formed per min has the same extinction on reaction with phenol reagent as 1 mmole of tyrosine Ref M.L.Anson J.gen Physiol 22,79,1939 & 17,151,1933
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