Total RNA Isolation Kit-Cells and Tissues, 20 preps

DESCRIPTION:

The procedure represents a well established silica membrane technology with a spin column format for rapid, bind-wash and elute method to prepare high quality total RNA. Biological samples are first lysed and homogenized by using a single lysis buffer containing chaotropic ions which immediately inactivate RNases and create an appropriate binding condition, which favors adsorption of RNA to the silica membrane. Following different washes to remove salts metabolites and macromolecular cellular components pure RNA is eluted under low ionic strength conditions with RNase-free water.

The purified RNA is ready for use in downstream applications such as:

• cDNA Synthesis
• RT-PCR and Real-Time PCR
• Northern Blot
• Microarray

HIGHLIGHTS:

• High recovery of pure and intact RNA
• Quick and Simple.
• No DNA Contamination.
• No Phenol/Chloroform Extraction.
• Complete column based purification
• Ready-to-use RNA for any downstream application The kit provides sufficient reagents for 20 Preps.

OTHERS:

• 10X Erythrocyte Lysis Buffer – GeneiPure^TM RNA Isolation Kit- Blood
• SE buffer – GeneiPure^TM RNA Isolation Kit- Yeast
• Lyticase
• 1X TE buffer- GeneiPure^TM RNA Isolation Kit- Bacteria
• Proteinase K
• Lysozyme
• Nuclease Free water
• GeneiPure^TM Columns
• Collection Tubes
• Instruction Manual