TEV Protease (1U/ul)

₹39,750.00 + GST 5% off
₹37,762.50 + ₹6,797.25 GST
₹44,559.75 (Incl. of all taxes)
In stock
Overview
Specifications
Appearance (Form) Liquid
Appearance (Colour) Colourless
Appearance (Clarity) Clear
Purity Electrophoretic purity is 90% by SDS PAGE
Notes
  Description:
Recombinant TEV Protease is a highly site-specific cysteine protease, which is found in the Tobacco Etch Virus. Due to its sequence specificity, the TEV Protease is a very powerful reagent for the removal of fusion tags from recombinant proteins after protein purification. The enzyme has been genetically modified to increase its activity and resistance to autolysis. It consists of the 27kDa catalytic domain with an N-terminal polyhistidine tag.

TEV Protease specifically recognizes a seven amino acid sequence of the general form
Glu-X-X-Tyr-X-Glnâ(Gly/Ser), most commonly Glu-Asn-Leu-Tyr-Phe-GlnâGly, and cleaves between glutamine and glycine or serine.

TEV Protease can also be used to cleave the affinity tag from a fusion protein immobilized on the affinity resin. Following digestion, the TEV Protease can be easily removed from the cleavage reaction by affinity chromatography using the polyhistidine tag at the N-terminal of the protease.

Applications:
Removal of affinity tags from fusion protein
More Information
Product Code 86851-5000 Units
HSN Code 38220090
Brand SRL
Usually dispatched in 1 WEEK
Pack Size 5000 Units

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