TCBS Agar

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Overview

(Thiosulphate Citrate Bile Salts Sucrose Agar)

DESCRIPTION

Storage : 8 to 25°C (Cool & dry area)

Shelf life : 36 Months

Specifications

Appearance (Colour)

Light tan w/greenish cast

Appearance (Form)

Free flowing, homogeneous powder

Solubility

88.08 gm/liter

Solubility before autoclaving (Clarity)

Very slightly opalescent

Gel strength

Firm, comparable with 1.5% agar gel

pH (25°C)

8.6 ± 0.2

Prepared Medium Appearance after autoclaving (Clarity)

Very slightly opalescent

Prepared Medium Appearance after autoclaving (Colour)

Forest green

Cultural Response

Inoculate and incubate at 35°C for 18-24 hours

Organism

Vibrio parahaemolyticus ATCC 17802

Inoculum (cfu) 10-100

Growth : Good, Colony colour : Bluish green

Organism

Vibrio cholerae ATCC 15748

Inoculum (cfu) 10-100

Growth : Good, Colony colour : Yellow

Organism

Shigella flexneri ATCC 12022

Inoculum (cfu) 10-100

Growth : Inhibited

Organism

Escherichia coli ATCC 25922

Inoculum (cfu) 10-100

Growth : Inhibited

Applications :

For the selective isolation of Vibrio cholerae and Vibrio parahaemolyticus from a variety of clinical specimens.

Composition :

Ingredients

gm/lt.

Agar

15.00

Peptic digest of animal tissue

5.00

Pancreatic digest of casein

5.00

Sodium chloride

10.00

Yeast extract

5.00

Sodium citrate

10.00

Sodium thiosulphate

10.00

Ferric citrate

1.00

Sodium cholate

3.00

Oxgall

5.00

Sucrose

20.00

Bromothymol Blue

0.04

Thymol Blue

0.04

Directions :

  1. Add 88.08 gm powder to 1.0 litre distilled/deionized water and mix thoroughly.
    2. Gently heat and bring to boiling.
    3. Do not autoclave.

 

More Information
Product Code 86626-500 Gms
HSN Code 38210000
Brand SRL
Usually dispatched in 7-10 DAYS
Pack Size 500 Gms
Purity % Vibrio parahaemolyticus ATCC 17802, Vibrio cholerae ATCC 15748, Shigella flexneri ATCC 12022,Escherichia coli ATCC 25922

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(Thiosulphate Citrate Bile Salts Sucrose Agar)

DESCRIPTION

Storage : 8 to 25°C (Cool & dry area)

Shelf life : 36 Months

Specifications

Appearance (Colour)

Light tan w/greenish cast

Appearance (Form)

Free flowing, homogeneous powder

Solubility

88.08 gm/liter

Solubility before autoclaving (Clarity)

Very slightly opalescent

Gel strength

Firm, comparable with 1.5% agar gel

pH (25°C)

8.6 ± 0.2

Prepared Medium Appearance after autoclaving (Clarity)

Very slightly opalescent

Prepared Medium Appearance after autoclaving (Colour)

Forest green

Cultural Response

Inoculate and incubate at 35°C for 18-24 hours

Organism

Vibrio parahaemolyticus ATCC 17802

Inoculum (cfu) 10-100

Growth : Good, Colony colour : Bluish green

Organism

Vibrio cholerae ATCC 15748

Inoculum (cfu) 10-100

Growth : Good, Colony colour : Yellow

Organism

Shigella flexneri ATCC 12022

Inoculum (cfu) 10-100

Growth : Inhibited

Organism

Escherichia coli ATCC 25922

Inoculum (cfu) 10-100

Growth : Inhibited

Applications :

For the selective isolation of Vibrio cholerae and Vibrio parahaemolyticus from a variety of clinical specimens.

Composition :

Ingredients

gm/lt.

Agar

15.00

Peptic digest of animal tissue

5.00

Pancreatic digest of casein

5.00

Sodium chloride

10.00

Yeast extract

5.00

Sodium citrate

10.00

Sodium thiosulphate

10.00

Ferric citrate

1.00

Sodium cholate

3.00

Oxgall

5.00

Sucrose

20.00

Bromothymol Blue

0.04

Thymol Blue

0.04

Directions :

  1. Add 88.08 gm powder to 1.0 litre distilled/deionized water and mix thoroughly.
    2. Gently heat and bring to boiling.
    3. Do not autoclave.

 

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