T4 DNA Ligase (High Conc.) , 1000U/µl
₹13,761.00 + ₹2,476.98 GST
₹16,237.98 (Incl. of all taxes)
Description
T4 DNA ligase catalyses the linkage of adjacent 5′-phosphate and 3′-hydroxy ends of double stranded DNA by the formation of phosphodiester bond.
Unit Definition :
One cohesive end ligase unit is defined as the amount of enzyme required to give 50% ligation of Lambda/Hind III digest in 30 minutes at 16°C in 20 μl of the reaction mixture and a 5′ DNA termini concentration of 0.12 μM.
Relation to other Ligase Units :
One cohesive end Ligase unit approximately equals 0.015 ATP-PP exchange unit (Weiss Unit).Storage Buffer : 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA, 50mM KCl, 1 mM DTT, 50% Glycerol and 200 μg/ml Nuclease free BSA.
Storage : -20°C
Note :
T4 DNA Ligase is supplied with 1 vial each of Cohesive & Blunt end Buffer.For Buffer Composition refer Buffer Set for Ligation given on page no 8.
Application :
This enzyme seals nicks in double stranded DNA and covalently joins DNA fragments with cohesive ends or blunt ends. This enzyme does not act on single stranded DNA.
Performance Test :
1. T4 DNA Ligase is routinely used in-house for quality control checks of all our cohesive and blunt end generating restriction enzymes. It is also used in the Blue-White standard assay.
2. Enzyme is tested for its performance in cloning experiments. Blunt End Ligation of λHae III blunt ended fragments using 1 μl of T4 DNA ligase at 22°C at different time points from 0-60 minutes.
| Product Code | 1160100021730-MME1HC |
|---|---|
| HSN Code | 35079099 |
| Brand | GeNei |
| Usually dispatched in | 2-3 WEEKS |
| Pack Size | 100000 U |
Description
T4 DNA ligase catalyses the linkage of adjacent 5′-phosphate and 3′-hydroxy ends of double stranded DNA by the formation of phosphodiester bond.
Unit Definition :
One cohesive end ligase unit is defined as the amount of enzyme required to give 50% ligation of Lambda/Hind III digest in 30 minutes at 16°C in 20 μl of the reaction mixture and a 5′ DNA termini concentration of 0.12 μM.
Relation to other Ligase Units :
One cohesive end Ligase unit approximately equals 0.015 ATP-PP exchange unit (Weiss Unit).Storage Buffer : 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA, 50mM KCl, 1 mM DTT, 50% Glycerol and 200 μg/ml Nuclease free BSA.
Storage : -20°C
Note :
T4 DNA Ligase is supplied with 1 vial each of Cohesive & Blunt end Buffer.For Buffer Composition refer Buffer Set for Ligation given on page no 8.
Application :
This enzyme seals nicks in double stranded DNA and covalently joins DNA fragments with cohesive ends or blunt ends. This enzyme does not act on single stranded DNA.
Performance Test :
1. T4 DNA Ligase is routinely used in-house for quality control checks of all our cohesive and blunt end generating restriction enzymes. It is also used in the Blue-White standard assay.
2. Enzyme is tested for its performance in cloning experiments. Blunt End Ligation of λHae III blunt ended fragments using 1 μl of T4 DNA ligase at 22°C at different time points from 0-60 minutes.