SS Agar (Salmonella Shigella Agar)

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Overview

DESCRIPTION

Storage : 8 to 25°C (Cool & dry area)

Shelf life : 36 Months

Specifications

Appearance (Colour)

Pinkish yellow

Appearance (Form)

Free flowing, homogeneous powder

Solubility

60.25gm/liter

Solubility before autoclaving (Clarity)

Clear to very slightly opalescent

Gel strength

Firm, comparable with 1.5% agar gel

pH (25°C)

7.0 ± 0.2

Prepared Medium Appearance after autoclaving (Clarity)

Clear to very slightly opalescent

Prepared Medium Appearance after autoclaving (Colour)

Reddish orange

Cultural Response

Inoculate and incubate at 37°C for 18 - 24 hours

Organism

Salmonella typhimurium ATCC 14028

Inoculum (cfu) 10-100

Growth : Good, Recovery Rate : >=50%, Colony colour : Colourless with black centres

Organism

Salmonella typhi ATCC 6539

Inoculum (cfu) 10-100

Growth : Good, Recovery Rate : >=50%, Colony colour : Colourless with black centres

Organism

Escherichia coli ATCC 25922

Inoculum (cfu) 10-100

Growth : Good, Recovery Rate : >=30%, Colony colour : Pink with bile precipitate

Organism

Proteus mirabilis ATCC 25933

Inoculum (cfu) 10-100

Growth : Good, Recovery Rate : >=40%, Colony colour : Colourless may have black centres

Organism

Shigella flexneri ATCC 12022

Inoculum (cfu) 10-100

Growth : Good, Recovery Rate : >=40%, Colony colour : Colourless

Organism

Enterobacter aerogenes ATCC 13048

Inoculum (cfu) 10-100

Growth : Good, Recovery Rate : >=30%, Colony colour : Cream pink

Organism

Enterococcus faecalis ATCC 29212

Inoculum

Growth : Inhibited

Applications :

For the selective isolation and differentiation of pathogenic enteric bacilli especially Salmonella and Shigella species.

Composition :

Ingredients

gm/lt.

Peptone

2.50

Agar

13.50

Lactose

10.00

Sodium thiosulphate

8.50

Sodium citrate

8.50

Beef extract(ex. buffalo)

5.00

Bile salts

8.50

Tryptone

2.50

Ferric citrate

1.00

Brilliant Green

0.00033

Neutral Red

0.025

Directions :

  1. Add 60.25 gm powder to 1.0 liter of distilled/deionized water and mix thoroughly.
    2. Gently heat and bring to boiling.
    3. Do not autoclave or overheat.

 

More Information
Product Code 39930-500 Gms
HSN Code 38210000
Brand SRL
Usually dispatched in 7-10 DAYS
Pack Size 500 Gms
Purity % Salmonella typhimurium ATCC 14028, Salmonella typhi ATCC 6539, Escherichia coli ATCC 25922, Proteus mirabilis ATCC 25933, Shigella flexneri ATCC 12022, Enterobacter aerogenes ATCC 13048, Enterococcus faecalis ATCC 29212

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DESCRIPTION

Storage : 8 to 25°C (Cool & dry area)

Shelf life : 36 Months

Specifications

Appearance (Colour)

Pinkish yellow

Appearance (Form)

Free flowing, homogeneous powder

Solubility

60.25gm/liter

Solubility before autoclaving (Clarity)

Clear to very slightly opalescent

Gel strength

Firm, comparable with 1.5% agar gel

pH (25°C)

7.0 ± 0.2

Prepared Medium Appearance after autoclaving (Clarity)

Clear to very slightly opalescent

Prepared Medium Appearance after autoclaving (Colour)

Reddish orange

Cultural Response

Inoculate and incubate at 37°C for 18 - 24 hours

Organism

Salmonella typhimurium ATCC 14028

Inoculum (cfu) 10-100

Growth : Good, Recovery Rate : >=50%, Colony colour : Colourless with black centres

Organism

Salmonella typhi ATCC 6539

Inoculum (cfu) 10-100

Growth : Good, Recovery Rate : >=50%, Colony colour : Colourless with black centres

Organism

Escherichia coli ATCC 25922

Inoculum (cfu) 10-100

Growth : Good, Recovery Rate : >=30%, Colony colour : Pink with bile precipitate

Organism

Proteus mirabilis ATCC 25933

Inoculum (cfu) 10-100

Growth : Good, Recovery Rate : >=40%, Colony colour : Colourless may have black centres

Organism

Shigella flexneri ATCC 12022

Inoculum (cfu) 10-100

Growth : Good, Recovery Rate : >=40%, Colony colour : Colourless

Organism

Enterobacter aerogenes ATCC 13048

Inoculum (cfu) 10-100

Growth : Good, Recovery Rate : >=30%, Colony colour : Cream pink

Organism

Enterococcus faecalis ATCC 29212

Inoculum

Growth : Inhibited

Applications :

For the selective isolation and differentiation of pathogenic enteric bacilli especially Salmonella and Shigella species.

Composition :

Ingredients

gm/lt.

Peptone

2.50

Agar

13.50

Lactose

10.00

Sodium thiosulphate

8.50

Sodium citrate

8.50

Beef extract(ex. buffalo)

5.00

Bile salts

8.50

Tryptone

2.50

Ferric citrate

1.00

Brilliant Green

0.00033

Neutral Red

0.025

Directions :

  1. Add 60.25 gm powder to 1.0 liter of distilled/deionized water and mix thoroughly.
    2. Gently heat and bring to boiling.
    3. Do not autoclave or overheat.

 

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