Southern Hybridization Teaching Kit, 5 expts.
₹12,588.80 (Incl. of all taxes)
DESCRIPTION:
Southern Hybridization is a technique involving transfer of DNA fragments separated on electrophoretic gels to nylon membrane. This is a widely used technique for detection of specific base sequences using complementary probes. This method is used to identify and quantitate specific DNA sequences.
The kit demonstrates the technique of Southern blotting and hybridization. The steps involved are:
- Electrophoretic separation of DNA molecules on agarose gel
- Electrophoretic transfer of DNA from gel to Nylon membrane
- Immobilization of DNA on to nylon membrane
- Hybridization and non-isotopic detection of DNA of interest.
The kit provides enough materials for 5 experiments.
MATERIALS PROVIDED:
- DNA marker
- Biotinylated Probe
- Hybridization Buffer
- Wash Buffers (4 nos.)
- Blocking Buffer
- Blocking Powder
- Streptavidin HRP Conjugate and Substrate
- Nylon Membrane
- Electro-transfer buffer
- Agarose
- Filter Paper
- Instruction Manual
| Product Code | 6109600011730_KT96A |
|---|---|
| HSN Code | 38220090 |
| Brand | GeNei |
| Usually dispatched in | 1-2 weeks |
| Pack Size | 1EA |
DESCRIPTION:
Southern Hybridization is a technique involving transfer of DNA fragments separated on electrophoretic gels to nylon membrane. This is a widely used technique for detection of specific base sequences using complementary probes. This method is used to identify and quantitate specific DNA sequences.
The kit demonstrates the technique of Southern blotting and hybridization. The steps involved are:
- Electrophoretic separation of DNA molecules on agarose gel
- Electrophoretic transfer of DNA from gel to Nylon membrane
- Immobilization of DNA on to nylon membrane
- Hybridization and non-isotopic detection of DNA of interest.
The kit provides enough materials for 5 experiments.
MATERIALS PROVIDED:
- DNA marker
- Biotinylated Probe
- Hybridization Buffer
- Wash Buffers (4 nos.)
- Blocking Buffer
- Blocking Powder
- Streptavidin HRP Conjugate and Substrate
- Nylon Membrane
- Electro-transfer buffer
- Agarose
- Filter Paper
- Instruction Manual