Simmon`s Citrate Agar
₹920.00 + ₹165.60 GST
₹1,085.60 (Incl. of all taxes)
DESCRIPTION
Storage : 8 to 25°C (Cool & dry area)
Shelf life : 36 Months
Specifications
| Appearance (Colour) | Mustard yellow |
| Appearance (Form) | Free flowing, homogeneous powder |
| Solubility | 24.28 gm/liter |
| Solubility before autoclaving (Clarity) | Slightly opalescent |
| pH (25°C) | 6.9 ± 0.2 |
| Prepared Medium Appearance after autoclaving (Clarity) | Clear to slightly opalescent |
| Prepared Medium Appearance after autoclaving (Colour) | Forest green |
| Cultural Response | Inoculate and incubate at 37°C for 18 - 24 hours |
| Organism | Salmonella typhimurium ATCC 14028 |
| Inoculum (cfu) 10-100 | Growth : Good |
| Organism | Enterobacter aerogenes ATCC 13048 |
| Inoculum (cfu) 10-100 | Growth : Good |
| Organism | Salmonella enteritidis ATCC 13076 |
| Inoculum (cfu) 10-100 | Growth : Good |
| Organism | Salmonella typhi ATCC 6539 |
| Inoculum (cfu) 10-100 | Growth : Fair-good |
| Organism | Escherichia coli ATCC 25922 |
| Inoculum | >=103,Growth : Inhibited |
| Organism | Shigella dysenteriae ATCC 13313 |
| Inoculum | >=103,Growth : Inhibited |
Applications :
For the differentiation of gram-negative bacteria on the basis of citrate utilization.
Composition :
| Ingredients | gm/lt. |
| Agar | 15.00 |
| Sodium chloride | 5.00 |
| Sodium citrate | 2.00 |
| K2HPO4 | 1.00 |
| (NH4)H2PO4 | 1.00 |
| MgSO4.H2O | 0.20 |
| Bromothymol Blue | 0.08 |
Directions :
- Add 24.28 gm powder to 1.0 liter of distilled/deionized water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Autoclave at 15 psi pressure at 121°C for 15 minutes.
| Product Code | 59437-100 Gms |
|---|---|
| HSN Code | 38210000 |
| Brand | SRL |
| Usually dispatched in | 7-10 DAYS |
| Pack Size | 100 Gms |
| Purity % | Salmonella typhimurium ATCC 14028, Enterobacter aerogenes ATCC 13048, Salmonella enteritidis ATCC 13076, Salmonella typhi ATCC 6539, Escherichia coli ATCC 25922, Shigella dysenteriae ATCC 13313 |
DESCRIPTION
Storage : 8 to 25°C (Cool & dry area)
Shelf life : 36 Months
Specifications
| Appearance (Colour) | Mustard yellow |
| Appearance (Form) | Free flowing, homogeneous powder |
| Solubility | 24.28 gm/liter |
| Solubility before autoclaving (Clarity) | Slightly opalescent |
| pH (25°C) | 6.9 ± 0.2 |
| Prepared Medium Appearance after autoclaving (Clarity) | Clear to slightly opalescent |
| Prepared Medium Appearance after autoclaving (Colour) | Forest green |
| Cultural Response | Inoculate and incubate at 37°C for 18 - 24 hours |
| Organism | Salmonella typhimurium ATCC 14028 |
| Inoculum (cfu) 10-100 | Growth : Good |
| Organism | Enterobacter aerogenes ATCC 13048 |
| Inoculum (cfu) 10-100 | Growth : Good |
| Organism | Salmonella enteritidis ATCC 13076 |
| Inoculum (cfu) 10-100 | Growth : Good |
| Organism | Salmonella typhi ATCC 6539 |
| Inoculum (cfu) 10-100 | Growth : Fair-good |
| Organism | Escherichia coli ATCC 25922 |
| Inoculum | >=103,Growth : Inhibited |
| Organism | Shigella dysenteriae ATCC 13313 |
| Inoculum | >=103,Growth : Inhibited |
Applications :
For the differentiation of gram-negative bacteria on the basis of citrate utilization.
Composition :
| Ingredients | gm/lt. |
| Agar | 15.00 |
| Sodium chloride | 5.00 |
| Sodium citrate | 2.00 |
| K2HPO4 | 1.00 |
| (NH4)H2PO4 | 1.00 |
| MgSO4.H2O | 0.20 |
| Bromothymol Blue | 0.08 |
Directions :
- Add 24.28 gm powder to 1.0 liter of distilled/deionized water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Autoclave at 15 psi pressure at 121°C for 15 minutes.