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SDS PAGE Sample Buffer (5X) (Reducing) (Laemmli Buffer 5X)

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₹1,800.00 + GST 5% off
₹1,710.00 + ₹205.20 GST
₹1,915.20 (Incl. of all taxes)
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Overview
Specifications
Appearance (Form) Liquid
Appearance (Colour) Dark Blue
Appearance (Clarity) Clear
Concentration 5X
pH of solution 6.8
SDS-PAGE Analysis Good resolution of individual proteins and optimum visibility of the tracking dye during electrophoresis.
Notes
  Description:
A ready-to-use 5X solution with beta-mercaptoethanol as a reducing agent. Used in SDS-PAGE for loading of conventional proteins. The use of Laemmli sample buffer ensures optimal band resolution when preparing proteins for SDS-PAGE with Tris-glycine-SDS running buffer. Role of 5X SDS PAGE Sample buffer : 1 .Tris is a buffer and it’s pH plays an essential role in preserving peptide bonds from breaking apart.
2. Sodium dodecyl sulphate is an anionic detergent that helps in linearizing (by denaturing) the proteins and bringing a net negative charge to the proteins irrespective of the initial charge.
3. The high density of glycerol ensures the sample moves down into the well.
4. Beta-mercaptoethanol, along with SDS, ensure the bands are individual polypeptide instead of molecular complexes.
5.The dye visually indicates the location (tracking) of the sample in the gel.

Recommended volume:
Working concentration of 1X ( 1:4)

Notes:
Mercaptoethanol is irritating and toxic if swallowed or inhaled, so it is advised to handle it in an active fumigation hood. Heating simply speeds up the process of denaturation by increasing molecular motion. Insufficient heating can leave some proteins incompletely denatured. It may require trial and error to achieve the best results. If samples are heated without first mixing with sample buffer, they will indeed be denatured, but not in the intended manner.
More Information
Product Code 99573-20 ml
HSN Code 38220090
Brand SRL
Usually dispatched in 2-3WEEK
Pack Size 20 ml
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  1. SDS PAGE Sample Buffer (5X) (Reducing) (Laemmli Buffer 5X)
    SDS PAGE Sample Buffer (5X) (Reducing) (Laemmli Buffer 5X)
    ₹1,800.00 + GST 5% off
    ₹1,710.00 + ₹205.20 GST
    ₹1,915.20 (Incl. of all taxes)
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Details
Specifications
Appearance (Form) Liquid
Appearance (Colour) Dark Blue
Appearance (Clarity) Clear
Concentration 5X
pH of solution 6.8
SDS-PAGE Analysis Good resolution of individual proteins and optimum visibility of the tracking dye during electrophoresis.
Notes
  Description:
A ready-to-use 5X solution with beta-mercaptoethanol as a reducing agent. Used in SDS-PAGE for loading of conventional proteins. The use of Laemmli sample buffer ensures optimal band resolution when preparing proteins for SDS-PAGE with Tris-glycine-SDS running buffer. Role of 5X SDS PAGE Sample buffer : 1 .Tris is a buffer and it’s pH plays an essential role in preserving peptide bonds from breaking apart.
2. Sodium dodecyl sulphate is an anionic detergent that helps in linearizing (by denaturing) the proteins and bringing a net negative charge to the proteins irrespective of the initial charge.
3. The high density of glycerol ensures the sample moves down into the well.
4. Beta-mercaptoethanol, along with SDS, ensure the bands are individual polypeptide instead of molecular complexes.
5.The dye visually indicates the location (tracking) of the sample in the gel.

Recommended volume:
Working concentration of 1X ( 1:4)

Notes:
Mercaptoethanol is irritating and toxic if swallowed or inhaled, so it is advised to handle it in an active fumigation hood. Heating simply speeds up the process of denaturation by increasing molecular motion. Insufficient heating can leave some proteins incompletely denatured. It may require trial and error to achieve the best results. If samples are heated without first mixing with sample buffer, they will indeed be denatured, but not in the intended manner.
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