RNaseA (DNase free)
₹14,013.00 + ₹2,522.34 GST
₹16,535.34 (Incl. of all taxes)
Description
Ribonuclease A (bovine pancreas) is used to hydrolyse RNA during isolation of DNA. Supplied as lyophilized powder which dissolves readily in analytical grade water. Essentially DNase free and > 95% pure as checked by ion exchange chromatography.
Activity :
In the range of 80 – 90 u/mg protein. Unit definition: One unit is that amount of enzyme causing hydrolysis of RNA at a rate such that k (velocity constant) equals unity at 25°C and pH 5.0.
Storage : -20°C
Application :
•In the isolation of DNA for RNA-free DNA.
•As a molecular weight marker.
Performance Test :
Efficiency tested in plasmid preparation by alkali lysis. DNase is not detected.
Absence of DNase contamination :
Varying units of RNase A (DNase Free) is incubated with 1 μg of substrate DNA at the recommended assay conditions in 50 μl reaction volume for 4 hours. A sharp unaltered banding pattern is an indication of the purity of RNase A (DNase Free) free from detectable non-specific nucleases.
| Product Code | 2150481001730-FC25J |
|---|---|
| HSN Code | 35079099 |
| Brand | GeNei |
| Usually dispatched in | 2-3 WEEKS |
| Pack Size | 100MG |
Description
Ribonuclease A (bovine pancreas) is used to hydrolyse RNA during isolation of DNA. Supplied as lyophilized powder which dissolves readily in analytical grade water. Essentially DNase free and > 95% pure as checked by ion exchange chromatography.
Activity :
In the range of 80 – 90 u/mg protein. Unit definition: One unit is that amount of enzyme causing hydrolysis of RNA at a rate such that k (velocity constant) equals unity at 25°C and pH 5.0.
Storage : -20°C
Application :
•In the isolation of DNA for RNA-free DNA.
•As a molecular weight marker.
Performance Test :
Efficiency tested in plasmid preparation by alkali lysis. DNase is not detected.
Absence of DNase contamination :
Varying units of RNase A (DNase Free) is incubated with 1 μg of substrate DNA at the recommended assay conditions in 50 μl reaction volume for 4 hours. A sharp unaltered banding pattern is an indication of the purity of RNase A (DNase Free) free from detectable non-specific nucleases.