Ribonuclease H (RNase H) (5U/ul)
Special Price
₹25,469.12
(Incl. of all taxes)
GST
₹26,809.60
₹22,720.00 + GST
5% off
₹21,584.00
+ ₹3,885.12 GST
₹25,469.12 (Incl. of all taxes)
Overview
| Specifications |
| Appearance (Form) | Liquid | | Appearance (Colour) | Colourless | | Appearance (Clarity) | Clear | | DNase activity | None detected | | Protease activity | None detected | | Concentration | 5U/μl | |
| |
| | Description:
RNase H is a 18.9 kDa recombinant endoribonuclease purified from an Escherichia coli strain, which over-expresses cloned RNase H gene (rnh). The enzyme hydrolyses specifically the phosphodiester bonds of RNA hybridized to DNA and produces 5´ phosphate-terminated oligoribonucleotides and single-stranded DNA.
Rnase H does not degrade single and double-stranded DNA or unhybridized RNA. It is a key enzyme in the removal of mRNA after first-strand cDNA synthesis. Treating cDNA with RNase H prior to PCR can improve sensitivity as RNA bonded to the cDNA template may prevent binding of the amplification primers in a PCR reaction. RNase H treatment is often necessary when amplifying longer, full-length cDNA targets. In addition, RNase H is useful for the removal of poly(A) tails on mRNAs after hybridization with oligo(dT) and also for the site-specific enzymatic cleavage of RNA.
Includes:
250U
RNAse H (5U/µl) - 50µl
10 X RNase H Reaction Buffer - 200 µl
Applications:
Removal of RNA after first strand cDNA synthesis (RT-PCR and qRT-PCR)
Removal of mRNA prior to synthesis of second strand cDNA
Removal of the poly(A) sequences of mRNA after hybridization with oligo(dT)
Site-specific cleavage of RNA
Studies of in vitro polyadenylation reaction products | |
More Information
| Product Code |
38080-250 Units |
| HSN Code |
35079099 |
| Brand |
SRL |
| Usually dispatched in |
2-3WEEK |
| Pack Size |
250 Units |
