Restriction Digestion Kit (Teaching)
Special Price
₹4,233.60
(Incl. of all taxes)
GST
₹4,704.00
₹4,200.00 + GST
10% off
₹3,780.00
+ ₹453.60 GST
₹4,233.60 (Incl. of all taxes)
Overview
| Specifications |
| Appearance (Form) | Liquid | | DNA Digestion | DNA was digested with the two restriction enzymes at 37°C in a period of one hour | | DNA degradation | DNA was incubated at 37°C for 24 hours | |
| |
| | Description:
The aim of the experiment is to cleave a given DNA specifically at particular sequences using restriction enzyme. This simple but powerful technique has been the mainstay in experimental molecular biology of DNA to generate fragments of defined sizes and having proper ends for cloning, release cloned fragments of DNA, characterizing a given DNA by restriction mapping and use such a map to differentiate between closely and distantly related genes or DNA.
In this experiment, use of restriction enzymes, in restriction mapping of lambda DNA by digestion with two enzymes Hind III and EcoRI is demonstrated.
| Includes | 10 Experiments | 50 Experiments | | Lambda DNA(250 ng/μl) | 160 μl | 1.0ml | | Agarose | 1 gm | 1.5 gm | | 50 X TAE stock Buffer | 25 ml | 50 ml | | Ethidium Bromide | 30 μl | 100 μl | | 6 X Gel loading Dye | 200 μl | 500 μl | | 10 X Assay buffer for Hind III | 100 μl | 250 μl | | 10 X Assay buffer for EcoR I | 100 μl | 250 μl | | Hi - Range marker | 60 μl | 150 μl | | Sterile Water | 500 μl | 1.5 ml | | EcoRI (1U/3μl) | 100 μl | 200 μl | | Hind III (1U/3μl) | 100 μl | 200 μl | | |
More Information
| Product Code |
18450-10 expt. Kit |
| HSN Code |
38229090 |
| Brand |
SRL |
| Usually dispatched in |
2-3WEEK |
| Pack Size |
10 expt. Kit |
