Reinforced Clostridial Agar

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In stock
Overview

DESCRIPTION

Storage : 8 to 25°C (Cool & dry area)

Shelf life : 36 Months

Specifications

Appearance (Colour)

Light yellow

Appearance (Form)

Free flowing, homogeneous powder

Solubility

51.00 gm/liter

Solubility before autoclaving (Clarity)

Clear to slightly opalescent

Gel strength

Firm, comparable with 1.35% agar gel

pH (25°C)

6.8 ± 0.2

Prepared Medium Appearance after autoclaving (Clarity)

Clear to very slightly opalescent

Prepared Medium Appearance after autoclaving (Colour)

Light yellow

Cultural Response

Inoculate and incubate at 35 ± 2°C for 40-48 hours, under anaerobic conditions

Organism

Clostridium perfringens ATCC 13124

Inoculum (cfu) 10-100

Growth : Good
Recovery Rate : >=50.0%

Organism

Bacteroides vulgatus ATCC 8482

Inoculum (cfu) 10-100

Growth : Good
Recovery Rate : >=50.0%

Organism

Bacteroides fragilis ATCC 23745

Inoculum (cfu) 10-100

Growth : Good
Recovery Rate : >=50.0%

Organism

Clostridium butyricum ATCC 9690

Inoculum (cfu) 10-100

Growth : Good
Recovery Rate : >=50.0%

Applications :

For the cultivation and differentiation of bacteria based on their ability to decarboxylate lysine, arginine and produce H2S.Recommended by APHA for isolation and identification of Yersinia from milk and milk products.

Composition :

Ingredients

gms/lt.

L-lysine

10.00

Peptic digest of animal tissue

5.00

Yeast extract

3.00

L-Arginine

10.00

Dextrose

1.00

Ferric ammonium citrate

0.50

Sodium thiosulphate

0.04

Bromocresol Purple

0.02

Agar

15.00

Directions :

  1. Add 44.56 gm powder to distilled/deionized water.
    2. Bring volume to 1.0 litre and mix thoroughly.
    3. Gently heat and bring to boiling.
    4. Autoclave at 15 psi pressure at 121°C for 15 minutes.
    5. Dispense as desired,preferably in slants with deep butts.

 

More Information
Product Code 78777-500 Gms
HSN Code 38210000
Brand SRL
Usually dispatched in 7-10 DAYS
Pack Size 500 Gms
Purity % Clostridium perfringens ATCC 13124, Bacteroides vulgatus ATCC 8482, Bacteroides fragilis ATCC 23745, Clostridium butyricum ATCC 9690

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DESCRIPTION

Storage : 8 to 25°C (Cool & dry area)

Shelf life : 36 Months

Specifications

Appearance (Colour)

Light yellow

Appearance (Form)

Free flowing, homogeneous powder

Solubility

51.00 gm/liter

Solubility before autoclaving (Clarity)

Clear to slightly opalescent

Gel strength

Firm, comparable with 1.35% agar gel

pH (25°C)

6.8 ± 0.2

Prepared Medium Appearance after autoclaving (Clarity)

Clear to very slightly opalescent

Prepared Medium Appearance after autoclaving (Colour)

Light yellow

Cultural Response

Inoculate and incubate at 35 ± 2°C for 40-48 hours, under anaerobic conditions

Organism

Clostridium perfringens ATCC 13124

Inoculum (cfu) 10-100

Growth : Good
Recovery Rate : >=50.0%

Organism

Bacteroides vulgatus ATCC 8482

Inoculum (cfu) 10-100

Growth : Good
Recovery Rate : >=50.0%

Organism

Bacteroides fragilis ATCC 23745

Inoculum (cfu) 10-100

Growth : Good
Recovery Rate : >=50.0%

Organism

Clostridium butyricum ATCC 9690

Inoculum (cfu) 10-100

Growth : Good
Recovery Rate : >=50.0%

Applications :

For the cultivation and differentiation of bacteria based on their ability to decarboxylate lysine, arginine and produce H2S.Recommended by APHA for isolation and identification of Yersinia from milk and milk products.

Composition :

Ingredients

gms/lt.

L-lysine

10.00

Peptic digest of animal tissue

5.00

Yeast extract

3.00

L-Arginine

10.00

Dextrose

1.00

Ferric ammonium citrate

0.50

Sodium thiosulphate

0.04

Bromocresol Purple

0.02

Agar

15.00

Directions :

  1. Add 44.56 gm powder to distilled/deionized water.
    2. Bring volume to 1.0 litre and mix thoroughly.
    3. Gently heat and bring to boiling.
    4. Autoclave at 15 psi pressure at 121°C for 15 minutes.
    5. Dispense as desired,preferably in slants with deep butts.

 

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