Rappaport Vassiliadis Salmonella Enrichment Broth, Harmonized
₹3,496.00 + ₹629.28 GST
₹4,125.28 (Incl. of all taxes)
DESCRIPTION
Storage : 8 to 25°C (Cool & dry area)
Shelf life : 36 Months
Specifications
| Appearance (Colour) | Pale green |
| Appearance (Form) | Free flowing, homogeneous powder |
| Solubility | 42.53 gm/liter |
| Solubility before autoclaving (Clarity) | Clear to slightly opalescent |
| pH (25°C) | 5.2 ± 0.2 |
| Prepared Medium Appearance after autoclaving (Clarity) | Clear to very slightly opalescent, may have a slight precipitate |
| Prepared Medium Appearance after autoclaving (Colour) | Dark turquoise |
| Cultural Response | Incubate aerobically in Rappaport Vassiliadis Salmonella Enrichment Broth,Harmonized at 41 ± 0.5°C for 21-27 hours. After incubation organisms are subcultured on XLD Agar (70791) and incubated at 35 ± 2°C for 18-24 hours |
| Organism | Salmonella typhimurium ATCC 14028 |
| Inoculum (cfu) 10-100 | Growth in Broth : Good |
| Organism | Salmonella enteritidis ATCC 13076 |
| Inoculum (cfu) 10-100 | Growth in Broth : Good |
| Organism | Escherichia coli ATCC 25922 |
| Inoculum (cfu) 10-100 | Growth in Broth : None-poor |
| Organism | Pseudomonas aeruginosa ATCC 27853 |
| Inoculum (cfu) 10-100 | Growth in Broth : None-poor |
| Organism | Enterococcus faecalis ATCC 29212 |
| Inoculum | Growth in Broth : Inhibited |
| Organism | Staphylococcus aureus ATCC 25923 |
| Inoculum | Growth in Broth : Inhibited |
Applications :
Used for selective enrichment of Salmonella species in pharmaceutical testing according to harmonized methods.
Composition :
| Ingredients | gm/lt. |
| Soyapeptone | 4.50 |
| Magnesium chloride hexahydrate | 29.00 |
| Sodium chloride | 8.00 |
| Dipotassium phosphate | 0.40 |
| Monopotassium phosphate | 0.60 |
| Malachite Green | 0.036 |
Directions :
- Add 42.53 gm powder to 1.0 liter of distilled/deionized water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Dispense into test tubes.
4. Autoclave at 15 psi pressure at 115°C for 15 minutes.
| Product Code | 59912-500 Gms |
|---|---|
| HSN Code | 38210000 |
| Brand | SRL |
| Usually dispatched in | 7-10 DAYS |
| Pack Size | 500 Gms |
| Purity % | Salmonella typhimurium ATCC 14028, Salmonella enteritidis ATCC 13076, Escherichia coli ATCC 25922, Pseudomos aeruginosa ATCC 27853, Enterococcus faecalis ATCC 29212, Staphylococcus aureus ATCC 25923 |
DESCRIPTION
Storage : 8 to 25°C (Cool & dry area)
Shelf life : 36 Months
Specifications
| Appearance (Colour) | Pale green |
| Appearance (Form) | Free flowing, homogeneous powder |
| Solubility | 42.53 gm/liter |
| Solubility before autoclaving (Clarity) | Clear to slightly opalescent |
| pH (25°C) | 5.2 ± 0.2 |
| Prepared Medium Appearance after autoclaving (Clarity) | Clear to very slightly opalescent, may have a slight precipitate |
| Prepared Medium Appearance after autoclaving (Colour) | Dark turquoise |
| Cultural Response | Incubate aerobically in Rappaport Vassiliadis Salmonella Enrichment Broth,Harmonized at 41 ± 0.5°C for 21-27 hours. After incubation organisms are subcultured on XLD Agar (70791) and incubated at 35 ± 2°C for 18-24 hours |
| Organism | Salmonella typhimurium ATCC 14028 |
| Inoculum (cfu) 10-100 | Growth in Broth : Good |
| Organism | Salmonella enteritidis ATCC 13076 |
| Inoculum (cfu) 10-100 | Growth in Broth : Good |
| Organism | Escherichia coli ATCC 25922 |
| Inoculum (cfu) 10-100 | Growth in Broth : None-poor |
| Organism | Pseudomonas aeruginosa ATCC 27853 |
| Inoculum (cfu) 10-100 | Growth in Broth : None-poor |
| Organism | Enterococcus faecalis ATCC 29212 |
| Inoculum | Growth in Broth : Inhibited |
| Organism | Staphylococcus aureus ATCC 25923 |
| Inoculum | Growth in Broth : Inhibited |
Applications :
Used for selective enrichment of Salmonella species in pharmaceutical testing according to harmonized methods.
Composition :
| Ingredients | gm/lt. |
| Soyapeptone | 4.50 |
| Magnesium chloride hexahydrate | 29.00 |
| Sodium chloride | 8.00 |
| Dipotassium phosphate | 0.40 |
| Monopotassium phosphate | 0.60 |
| Malachite Green | 0.036 |
Directions :
- Add 42.53 gm powder to 1.0 liter of distilled/deionized water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Dispense into test tubes.
4. Autoclave at 15 psi pressure at 115°C for 15 minutes.