Purification of His Tag fusion protein (NTA based) Kit, 4 preps
₹15,207.00 + ₹1,824.84 GST
₹17,031.84 (Incl. of all taxes)
DESCRIPTION:
His tag fusion protein purification kit is a cross linked agarose
based resin pre-charged with Ni2 ions. Binding of the protein
is based on the interaction between the polyhistidine tag of
the recombinant protein and immobilized Ni2 ions. Nickel is
chelated on CL-Agarose through nitrilotriacetic acid which
enables strong and efficient binding of target protein due to its
affinity for 6X His Tag. This enables rapid purification of
polyhistidine tag fusion protein from bacterial lysate.
Nitrilotriiacetic acid (NTA) is a tetra-dentate chelator which
occupies 4 of the 6 binding sites in the coordination sphere of
Ni2 ions. The remaining 2 coordination sites are usually
occupied by water molecules and can be exchanged with
histidine residues of the recombinant protein.
APPLICATION:
For purification of his-tagged proteins.
MATERIALS PROVIDED:
Nickel CL-Agarose column
2X Equilibration Buffer
2X Wash Buffer
2X Elution Buffer
Store: 4°C
| Product Code | 2160900011730-KT237_ |
|---|---|
| HSN Code | 38220090 |
| Brand | GeNei |
| Usually dispatched in | 2-3 WEEKS |
| Pack Size | 1 EA |
DESCRIPTION:
His tag fusion protein purification kit is a cross linked agarose
based resin pre-charged with Ni2 ions. Binding of the protein
is based on the interaction between the polyhistidine tag of
the recombinant protein and immobilized Ni2 ions. Nickel is
chelated on CL-Agarose through nitrilotriacetic acid which
enables strong and efficient binding of target protein due to its
affinity for 6X His Tag. This enables rapid purification of
polyhistidine tag fusion protein from bacterial lysate.
Nitrilotriiacetic acid (NTA) is a tetra-dentate chelator which
occupies 4 of the 6 binding sites in the coordination sphere of
Ni2 ions. The remaining 2 coordination sites are usually
occupied by water molecules and can be exchanged with
histidine residues of the recombinant protein.
APPLICATION:
For purification of his-tagged proteins.
MATERIALS PROVIDED:
Nickel CL-Agarose column
2X Equilibration Buffer
2X Wash Buffer
2X Elution Buffer
Store: 4°C