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PstI (10U/mcl)

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₹3,000.00 + GST 5% off
₹2,850.00 + ₹342.00 GST
₹3,192.00 (Incl. of all taxes)
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Overview
Specifications
Appearance (Form) Liquid
Appearance (Colour) Colourless
Appearance (Clarity) Clear
Activity One unit is defined as the amount of enzyme required to digest 1 μg of lambda DNA in 1 hour at 37 °C in 50 μL of assay buffer.
DNA degradation Incubation of single stranded and double stranded radiolabeled oligonucleotides with 10 units of the enzyme for 4 hours at 37 °C.
Star Acitvity After 160-fold overdigestion (10 U/μg Lambda DNA for 16 hours at 37 °C) with enzyme the fragmentation pattern is analysed. No detectable changes compared to the theoretical fragmentation pattern are considered as absence of star activity.
Notes
  Description:
PstI is an endonuclease enzyme isolated from strains of Providencia stuartii, recombinant, E.coli and is part of the restriction modification system.
In molecular biology it is used as a restriction enzyme. It creates sticky ends with 3' end overhangs. The nucleic acid sequence where the enzyme cuts is CTGCAG, which, as the complementary sequence is GACGTC, has rotational symmetry.

Includes:
1500U
• PstI - 150 μl
• 10X Reaction Buffer - 1.0ml
More Information
Product Code 94651-1500 Units
HSN Code 38229090
Brand SRL
Usually dispatched in 2-3WEEK
Pack Size 1500 Units
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  1. PstI (10U/mcl)
    PstI (10U/mcl)
    ₹3,000.00 + GST 5% off
    ₹2,850.00 + ₹342.00 GST
    ₹3,192.00 (Incl. of all taxes)
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Details
Specifications
Appearance (Form) Liquid
Appearance (Colour) Colourless
Appearance (Clarity) Clear
Activity One unit is defined as the amount of enzyme required to digest 1 μg of lambda DNA in 1 hour at 37 °C in 50 μL of assay buffer.
DNA degradation Incubation of single stranded and double stranded radiolabeled oligonucleotides with 10 units of the enzyme for 4 hours at 37 °C.
Star Acitvity After 160-fold overdigestion (10 U/μg Lambda DNA for 16 hours at 37 °C) with enzyme the fragmentation pattern is analysed. No detectable changes compared to the theoretical fragmentation pattern are considered as absence of star activity.
Notes
  Description:
PstI is an endonuclease enzyme isolated from strains of Providencia stuartii, recombinant, E.coli and is part of the restriction modification system.
In molecular biology it is used as a restriction enzyme. It creates sticky ends with 3' end overhangs. The nucleic acid sequence where the enzyme cuts is CTGCAG, which, as the complementary sequence is GACGTC, has rotational symmetry.

Includes:
1500U
• PstI - 150 μl
• 10X Reaction Buffer - 1.0ml
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