Plasmid Isolation Kit from Bacterial cells (Teaching)
Special Price
7,200.36
(Incl. of all taxes)
GST
8,000.40
₹6,780.00 + GST
10% off
₹6,102.00
+ ₹1,098.36 GST
₹7,200.36 (Incl. of all taxes)
Overview
| Specifications |
| Evaluation |
Components of the kit were tested for isolation of plasmid DNA from bacterial cells according to the protocol |
| Stability |
Cell pellets should yield plasmid DNA till the end of the expiration date |
| A260/A280 |
1.7 ~ 1.9 |
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Description:
The overall goal is to isolate extra-chromosomal, plasmid DNA from bacterial cells that contain the plasmid. The cells are first re-suspended in a suitable buffer (that minimizes nuclease activity) and ruptured to release the cellular contents. Subsequently, the plasmid DNA and RNA are separated from genomic DNA. Finally, by decreasing the “effective concentration” of water, the plasmid DNA is precipitated through sheer desolvation effects. If desired, the contaminating RNA can be removed by digestion with RNase A.
| Includes |
15 Experiments |
25 Experiments |
| Bacterial Cell Pellet |
18 vials |
28 vials |
| Solution I |
3 ml |
5 ml |
| 5 N NaOH |
400 μl |
750 μl |
| 10% SDS |
1 ml |
3 ml |
| Sterile water |
10 ml |
30 ml |
| Solution III |
4 ml |
10 ml |
| DNA precipitation solution |
14 ml |
25 ml |
| Wash solution |
14 ml |
25 ml |
| TE buffer |
500 μl |
750 μl |
| Gel loading dye |
100 μl |
200 μl |
| Agarose |
1.5 gm |
2.5 gm |
| Ethidium bromide |
30 μl |
75 μl |
| 50X TAE buffer |
25 ml |
50 ml |
| Plasmid control |
60 μl |
100 μl |
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More Information
| Product Code |
27725-15 expt. Kit |
| HSN Code |
38220090 |
| Brand |
SRL |
| Usually dispatched in |
1 WEEK |
| Pack Size |
15 expt. Kit |
