Phenylalanine Agar
₹1,104.00 + ₹198.72 GST
₹1,302.72 (Incl. of all taxes)
(Phenylalanine Deaminase Medium)
DESCRIPTION
Storage : 8 to 25°C (Cool & dry area)
Shelf life : 36 Months
Specifications
| Appearance (Colour) | Light tan |
| Appearance (Form) | Free flowing, homogeneous powder |
| Solubility | 26.00 gm/liter |
| Solubility before autoclaving (Clarity) | Slightly opalescent w/o significant precipitate |
| Gel strength | Firm, comparable with 1.5% agar gel |
| pH (25°C) | 7.3 ± 0.2 |
| Prepared Medium Appearance after autoclaving (Clarity) | Slightly opalescent w/o significant precipitate |
| Prepared Medium Appearance after autoclaving (Colour) | Light amber |
| Cultural Response | Inoculate and incubate at 35±2°C for 18-24 hours. After 24 hours of incubation observe the slants for growth. Then add 10% aqueous Ferric chloride reagent to the grown cultures |
| Organism | Proteus vulgaris ATCC 13315 |
| Inoculum (cfu) 10-100 | Growth : Good, Phenylalanine deaminase : (+) |
| Organism | Escherichia coli ATCC 25922 |
| Inoculum (cfu) 10-100 | Growth : Good, Phenylalanine deaminase : (-) |
| Organism | Enterobacter aerogenes ATCC 13048 |
| Inoculum (cfu) 10-100 | Growth : Good, Phenylalanine deaminase : (-) |
| Organism | Proteus mirabilis ATCC 25933 |
| Inoculum (cfu) 10-100 | Growth : Good, Phenylalanine deaminase : (+) |
| Key | Positive (+) : Dark green colour |
Applications :
For the differentiation of enteric gram- negative bacilli on the basis of their ability to produce phenyl pruvic acid from phenylalanine.
Composition :
| Ingredients | gm/lt. |
| Sodium chloride | 5.00 |
| Yeast extract | 3.00 |
| DL-Phenylalanine | 2.00 |
| Disodium hydrogen phosphate | 1.00 |
| Agar | 15.00 |
Directions :
- Add 26.00 gm powder to 1.0 litre distilled/deionized water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Dispense into test tubes.
4. Autoclave at 15 psi pressure at 121°C for 15 minutes.
5. Allow the tubes to solidify in a slanted position.
| Product Code | 92335-100 Gms |
|---|---|
| HSN Code | 38210000 |
| Brand | SRL |
| Usually dispatched in | 7-10 DAYS |
| Pack Size | 100 Gms |
| Purity % | Proteus vulgaris ATCC 13315, Escherichia coli ATCC 25922, Enterobacter aerogenes ATCC 13048, Proteus mirabilis ATCC 25933 |
(Phenylalanine Deaminase Medium)
DESCRIPTION
Storage : 8 to 25°C (Cool & dry area)
Shelf life : 36 Months
Specifications
| Appearance (Colour) | Light tan |
| Appearance (Form) | Free flowing, homogeneous powder |
| Solubility | 26.00 gm/liter |
| Solubility before autoclaving (Clarity) | Slightly opalescent w/o significant precipitate |
| Gel strength | Firm, comparable with 1.5% agar gel |
| pH (25°C) | 7.3 ± 0.2 |
| Prepared Medium Appearance after autoclaving (Clarity) | Slightly opalescent w/o significant precipitate |
| Prepared Medium Appearance after autoclaving (Colour) | Light amber |
| Cultural Response | Inoculate and incubate at 35±2°C for 18-24 hours. After 24 hours of incubation observe the slants for growth. Then add 10% aqueous Ferric chloride reagent to the grown cultures |
| Organism | Proteus vulgaris ATCC 13315 |
| Inoculum (cfu) 10-100 | Growth : Good, Phenylalanine deaminase : (+) |
| Organism | Escherichia coli ATCC 25922 |
| Inoculum (cfu) 10-100 | Growth : Good, Phenylalanine deaminase : (-) |
| Organism | Enterobacter aerogenes ATCC 13048 |
| Inoculum (cfu) 10-100 | Growth : Good, Phenylalanine deaminase : (-) |
| Organism | Proteus mirabilis ATCC 25933 |
| Inoculum (cfu) 10-100 | Growth : Good, Phenylalanine deaminase : (+) |
| Key | Positive (+) : Dark green colour |
Applications :
For the differentiation of enteric gram- negative bacilli on the basis of their ability to produce phenyl pruvic acid from phenylalanine.
Composition :
| Ingredients | gm/lt. |
| Sodium chloride | 5.00 |
| Yeast extract | 3.00 |
| DL-Phenylalanine | 2.00 |
| Disodium hydrogen phosphate | 1.00 |
| Agar | 15.00 |
Directions :
- Add 26.00 gm powder to 1.0 litre distilled/deionized water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Dispense into test tubes.
4. Autoclave at 15 psi pressure at 121°C for 15 minutes.
5. Allow the tubes to solidify in a slanted position.