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Pfu Mix (2x)

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₹7,330.00 + GST 5% off
₹6,963.50 + ₹835.62 GST
₹7,799.12 (Incl. of all taxes)
In stock
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Overview
Specifications
Appearance (Form) Liquid
Appearance (Colour) Blue
Appearance (Clarity) Clear
Amplification capability Upto 2kb size
Absence of nucleases No DNA degradation seen when incubated overnight with the Master mix.
DNA contamination No Plasmid or Genomic DNA contamination seen
Notes
  Description:
Pfu Mix(2x) is a premixed, ready-to-use solution containing Pfu DNA Polymerase, dNTP, MgSO4 and Reaction Buffer at optimal concentrations for efficient amplification of DNA templates by PCR. To prepare the final PCR, only primers and template DNA are added. Pfu Mix contributes to highly reproducible PCR by reducing the risk of pipetting errors, miscalculation and contamination. It also contributes to higher sensitivity by adding intensifier and optimizer.
Pfu DNA polymerase, derived from the hyperthermophilic archae Pyrococcus furiosus, has been shown to exhibit superior thermostability and proofreading properties compared to other thermostable polymerase. Unlike Taq DNA polymerase, highly thermostable Pfu DNA polymerase possesses 3' to 5' exonuclease proofreading activity that enables the polymerase to correct nucleotide-misincorporation errors. This means that Pfu DNA polymerase-generated PCR fragments will have fewer errors than Taq-generated PCR inserts. Using Pfu DNA polymerase in your PCR reactions results in blunt-ended PCR products, which are ideal for cloning into blunt-ended vectors. Pfu DNA polymerase is superior for techniques that require high-fidelity DNA synthesis.

Applications:
High fidelity PCR
High reproducible PCR
PCR cloning
Site-directed mutagenesis

Includes:
1ml (40 rxns)
• Pfu Mix (2x) -1.0 ml
• Water, Nuclease Free - 1.0ml

 

More Information
Product Code 94531-1 ml
HSN Code 38229090
Brand SRL
Usually dispatched in 2-3WEEK
Pack Size 1 ml
main product photo

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  1. Pfu Mix (2x)
    Pfu Mix (2x)
    ₹7,330.00 + GST 5% off
    ₹6,963.50 + ₹835.62 GST
    ₹7,799.12 (Incl. of all taxes)
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Details
Specifications
Appearance (Form) Liquid
Appearance (Colour) Blue
Appearance (Clarity) Clear
Amplification capability Upto 2kb size
Absence of nucleases No DNA degradation seen when incubated overnight with the Master mix.
DNA contamination No Plasmid or Genomic DNA contamination seen
Notes
  Description:
Pfu Mix(2x) is a premixed, ready-to-use solution containing Pfu DNA Polymerase, dNTP, MgSO4 and Reaction Buffer at optimal concentrations for efficient amplification of DNA templates by PCR. To prepare the final PCR, only primers and template DNA are added. Pfu Mix contributes to highly reproducible PCR by reducing the risk of pipetting errors, miscalculation and contamination. It also contributes to higher sensitivity by adding intensifier and optimizer.
Pfu DNA polymerase, derived from the hyperthermophilic archae Pyrococcus furiosus, has been shown to exhibit superior thermostability and proofreading properties compared to other thermostable polymerase. Unlike Taq DNA polymerase, highly thermostable Pfu DNA polymerase possesses 3' to 5' exonuclease proofreading activity that enables the polymerase to correct nucleotide-misincorporation errors. This means that Pfu DNA polymerase-generated PCR fragments will have fewer errors than Taq-generated PCR inserts. Using Pfu DNA polymerase in your PCR reactions results in blunt-ended PCR products, which are ideal for cloning into blunt-ended vectors. Pfu DNA polymerase is superior for techniques that require high-fidelity DNA synthesis.

Applications:
High fidelity PCR
High reproducible PCR
PCR cloning
Site-directed mutagenesis

Includes:
1ml (40 rxns)
• Pfu Mix (2x) -1.0 ml
• Water, Nuclease Free - 1.0ml

 

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