Pfu DNA Polymerase (5U/mcl)

₹6,480.00 + GST 5% off
₹6,156.00 + ₹738.72 GST
₹6,894.72 (Incl. of all taxes)
In stock
Overview
Specifications
Appearance (Form) Liquid
Appearance (Colour) Colourless
Appearance (Clarity) Clear
Amplification capability Up to 2kb DNA fragment
Absence of nucleases No DNA degradation seen proving absence of nuleases
DNA contamination No Genomic or Plasmid DNA contamination
Notes
  Description:
Pfu DNA polymerase, derived from the hyperthermophilic archae Pyrococcus furiosus, has been shown to exhibit superior thermostability and proofreading properties compared to other thermostable polymerase. Unlike Taq DNA polymerase, highly thermostable Pfu DNA polymerase possesses 3' to 5' exonuclease proofreading activity that enables the polymerase to correct nucleotide-misincorporation errors. This means that Pfu DNA polymerase-generated PCR fragments will have fewer errors than Taq-generated PCR inserts. Using Pfu DNA polymerase in your PCR reactions results in blunt-ended PCR products, which are ideal for cloning into blunt-ended vectors. Pfu DNA polymerase is superior for techniques that require high-fidelity DNA synthesis.

Applications:
High-fidelity PCR and primer-extension reactions
PCR cloning and blunt-ended amplification product generation

Includes:
250 Units
• Pfu TAQ Polymerase (5U/μl) - 50 μl
• 10X Pfu TAQ Buffer with MgCl2 - 1.25ml
• 6 X Loading Buffer - 1.0ml
More Information
Product Code 22774-250 Units
HSN Code 38229090
Brand SRL
Usually dispatched in 2-3WEEK
Pack Size 250 Units

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Specifications
Appearance (Form) Liquid
Appearance (Colour) Colourless
Appearance (Clarity) Clear
Amplification capability Up to 2kb DNA fragment
Absence of nucleases No DNA degradation seen proving absence of nuleases
DNA contamination No Genomic or Plasmid DNA contamination
Notes
  Description:
Pfu DNA polymerase, derived from the hyperthermophilic archae Pyrococcus furiosus, has been shown to exhibit superior thermostability and proofreading properties compared to other thermostable polymerase. Unlike Taq DNA polymerase, highly thermostable Pfu DNA polymerase possesses 3' to 5' exonuclease proofreading activity that enables the polymerase to correct nucleotide-misincorporation errors. This means that Pfu DNA polymerase-generated PCR fragments will have fewer errors than Taq-generated PCR inserts. Using Pfu DNA polymerase in your PCR reactions results in blunt-ended PCR products, which are ideal for cloning into blunt-ended vectors. Pfu DNA polymerase is superior for techniques that require high-fidelity DNA synthesis.

Applications:
High-fidelity PCR and primer-extension reactions
PCR cloning and blunt-ended amplification product generation

Includes:
250 Units
• Pfu TAQ Polymerase (5U/μl) - 50 μl
• 10X Pfu TAQ Buffer with MgCl2 - 1.25ml
• 6 X Loading Buffer - 1.0ml
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