Perfringens Agar Base ( O.P.S.P. )

₹5,200.00 + GST 8% off
₹4,784.00 + ₹861.12 GST
₹5,645.12 (Incl. of all taxes)
In stock
Overview

DESCRIPTION

Storage : 8 to 25°C (Cool & dry area)

Shelf life : 36 Months

Specifications

Appearance (Colour)

Yellow

Appearance (Form)

Free flowing, homogeneous powder

Solubility

50.50 gm/liter

Solubility before autoclaving (Clarity)

Clear to slightly opalescent

Gel strength

Firm, comparable with 1.5% agar gel

pH (25°C)

7.3 ± 0.2

Prepared Medium Appearance after autoclaving (Clarity)

Clear to slightly opalescent

Prepared Medium Appearance after autoclaving (Colour)

Amber

Cultural Response

Inoculate and incubate at 35-37°C for 18-48 hours

Organism

Clostridium perfringens ATCC 12924

Inoculum (cfu) 10-100

Growth : Good
Colony colour : Black

Organism

Enterococcus faecalis ATCC 29212

Inoculum (cfu) 10-100

Growth : None-poor
Colony colour : White

Organism

Bacillus subtilis ATCC 6633

Inoculum

Growth : Inhibited

Organism

Staphylococcus aureus ATCC 25923

Inoculum

Growth : Inhibited

Organism

Proteus vulgaris ATCC 13315

Inoculum

Growth : Inhibited

Applications :

For presumptive identification of Clostridium perfringens in foods.

Composition :

Ingredients

gm/lt.

Enzymatic digest of Casein

15.00

Papaic digest of soyabean meal

5.00

Yeast extract

5.00

Liver extract

7.00

Ferric ammonium citrate

1.00

Sodium metabisulphite

1.00

Tris buffer

1.50

Agar

15.00

Directions :

  1. Add 25.25 gm powder to 500 ml distilled/deionized water and mix thoroughly.
    2. Gently heat and bring to boiling.
    3. Autoclave at 15 psi pressure at 121°C for 15 minutes.
    4. Cool to 50 °C. Add aseptically Perfringens Supplement -I (11976) and Perfringens Supplement -II (62598) each or as desired.

 

More Information
Product Code 66916-500 Gms
HSN Code 38210000
Brand SRL
Usually dispatched in 7-10 DAYS
Pack Size 500 Gms
Purity % Clostridium perfringens ATCC 12924, Enterococcus faecalis ATCC 29212, Bacillus subtilis ATCC 6633, Staphylococcus aureus ATCC 25923, Proteus vulgaris ATCC 13315

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DESCRIPTION

Storage : 8 to 25°C (Cool & dry area)

Shelf life : 36 Months

Specifications

Appearance (Colour)

Yellow

Appearance (Form)

Free flowing, homogeneous powder

Solubility

50.50 gm/liter

Solubility before autoclaving (Clarity)

Clear to slightly opalescent

Gel strength

Firm, comparable with 1.5% agar gel

pH (25°C)

7.3 ± 0.2

Prepared Medium Appearance after autoclaving (Clarity)

Clear to slightly opalescent

Prepared Medium Appearance after autoclaving (Colour)

Amber

Cultural Response

Inoculate and incubate at 35-37°C for 18-48 hours

Organism

Clostridium perfringens ATCC 12924

Inoculum (cfu) 10-100

Growth : Good
Colony colour : Black

Organism

Enterococcus faecalis ATCC 29212

Inoculum (cfu) 10-100

Growth : None-poor
Colony colour : White

Organism

Bacillus subtilis ATCC 6633

Inoculum

Growth : Inhibited

Organism

Staphylococcus aureus ATCC 25923

Inoculum

Growth : Inhibited

Organism

Proteus vulgaris ATCC 13315

Inoculum

Growth : Inhibited

Applications :

For presumptive identification of Clostridium perfringens in foods.

Composition :

Ingredients

gm/lt.

Enzymatic digest of Casein

15.00

Papaic digest of soyabean meal

5.00

Yeast extract

5.00

Liver extract

7.00

Ferric ammonium citrate

1.00

Sodium metabisulphite

1.00

Tris buffer

1.50

Agar

15.00

Directions :

  1. Add 25.25 gm powder to 500 ml distilled/deionized water and mix thoroughly.
    2. Gently heat and bring to boiling.
    3. Autoclave at 15 psi pressure at 121°C for 15 minutes.
    4. Cool to 50 °C. Add aseptically Perfringens Supplement -I (11976) and Perfringens Supplement -II (62598) each or as desired.

 

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