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NotI (10U/mcl)

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₹4,800.00 + GST 5% off
₹4,560.00 + ₹547.20 GST
₹5,107.20 (Incl. of all taxes)
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Overview
Specifications
Appearance (Form) Liquid
Appearance (Colour) Colourless
Appearance (Clarity) Clear
Activity One unit is defined as the amount of enzyme required to digest 1 μg of lambda DNA in 1 hour at 37 °C in 50 μL of assay buffer
DNA degradation Incubation of single stranded and double stranded radiolabeled oligonucleotides with 10 units of the enzyme for 4 hours at 37 °C
Star Acitvity After 160-fold overdigestion (10 U/μg Lambda DNA for 16 hours at 37 °C) with enzyme the fragmentation pattern is analysed. No detectable changes compared to the theoretical fragmentation pattern are considered as absence of star activity
Notes
  Description:
NotI is an endonuclease enzyme isolated from strains of Nocardia otitidis-caviarum and is part of the restriction modification system.
In molecular biology it is used as a restriction enzyme. It creates sticky ends with a 3' overhang. The nucleic acid sequence where the enzyme cuts is GCGGCCGC, which, as the complementary sequence is CGCCGGCG, has rotational symmetry.

Includes:
100U
• NotI - 10 μl
• 10X Reaction Buffer - 1.0ml
More Information
Product Code 97361-100 Units
HSN Code 38229090
Brand SRL
Usually dispatched in 2-3WEEK
Pack Size 100 Units
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  1. NotI (10U/mcl)
    NotI (10U/mcl)
    ₹4,800.00 + GST 5% off
    ₹4,560.00 + ₹547.20 GST
    ₹5,107.20 (Incl. of all taxes)
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Details
Specifications
Appearance (Form) Liquid
Appearance (Colour) Colourless
Appearance (Clarity) Clear
Activity One unit is defined as the amount of enzyme required to digest 1 μg of lambda DNA in 1 hour at 37 °C in 50 μL of assay buffer
DNA degradation Incubation of single stranded and double stranded radiolabeled oligonucleotides with 10 units of the enzyme for 4 hours at 37 °C
Star Acitvity After 160-fold overdigestion (10 U/μg Lambda DNA for 16 hours at 37 °C) with enzyme the fragmentation pattern is analysed. No detectable changes compared to the theoretical fragmentation pattern are considered as absence of star activity
Notes
  Description:
NotI is an endonuclease enzyme isolated from strains of Nocardia otitidis-caviarum and is part of the restriction modification system.
In molecular biology it is used as a restriction enzyme. It creates sticky ends with a 3' overhang. The nucleic acid sequence where the enzyme cuts is GCGGCCGC, which, as the complementary sequence is CGCCGGCG, has rotational symmetry.

Includes:
100U
• NotI - 10 μl
• 10X Reaction Buffer - 1.0ml
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