Nickel - CL Agarose
₹9,558.00 + ₹1,720.44 GST
₹11,278.44 (Incl. of all taxes)
Description
His tag fusion protein purification kit is a cross linked agarose based resin pre-charged with Ni2 ions. Binding of the protein is based on the interaction between the polyhistidine tag of the recombinant protein and immobilized Ni2 ions. Nickel is chelated on CL-Agarose through Iminodiacetic acid which enables strong and efficient binding of target protein due to its affinity for 6X His Tag. This enables rapid purification of polyhistidine tag fusion protein from bacterial lysate. Iminodiacetic acid (IDA) is a three-dentate chelator which occupies 3 of the 6 binding sites in the coordination sphere of Ni2 ions. The remaining 3 coordination sites are usually occupied by water molecules and can be exchanged with histidine residues of the recombinant protein
Application:
For purification of his-tagged proteins.
Store: 4°C
| Product Code | 2110580051730-LIA37 |
|---|---|
| HSN Code | 13021915 |
| Brand | GeNei |
| Usually dispatched in | 2-3 WEEKS |
| Pack Size | 5ML |
Description
His tag fusion protein purification kit is a cross linked agarose based resin pre-charged with Ni2 ions. Binding of the protein is based on the interaction between the polyhistidine tag of the recombinant protein and immobilized Ni2 ions. Nickel is chelated on CL-Agarose through Iminodiacetic acid which enables strong and efficient binding of target protein due to its affinity for 6X His Tag. This enables rapid purification of polyhistidine tag fusion protein from bacterial lysate. Iminodiacetic acid (IDA) is a three-dentate chelator which occupies 3 of the 6 binding sites in the coordination sphere of Ni2 ions. The remaining 3 coordination sites are usually occupied by water molecules and can be exchanged with histidine residues of the recombinant protein
Application:
For purification of his-tagged proteins.
Store: 4°C