Mueller Kauffman Tetrathionate Novobiocin Broth Base (MKTTn)
₹3,864.00 + ₹695.52 GST
₹4,559.52 (Incl. of all taxes)
DESCRIPTION
Storage: 8 to 25°C (Cool & dry area)
Shelf life: 36 Months
Specifications
| Appearance (Colour) | Pale green |
| Appearance (Form) | Free flowing, homogeneous powder |
| Solubility | 89.50 gm/liter |
| Solubility before autoclaving (Clarity) | Opaque suspension which on standing gives a heavy white precipitate |
| pH (25°C) | 8.2 ± 0.2 |
| Prepared Medium Appearance after autoclaving (Clarity) | Opaque suspension which on standing gives a heavy white precipitate |
| Prepared Medium Appearance after autoclaving (Colour) | Pale green |
| Cultural Response | Inoculate and incubate at 43°C for 18-48 hours, with added 20ml iodine solution and MKTT Novobiocin Supplement (31601), when subcultured on Soyabean Casein Digest Agar (33291) |
| Organism | Proteus vulgaris ATCC 13315 |
| Inoculum (cfu) 10-100 | Growth : None - poor |
| Organism | Salmonella enteritidis ATCC 13076 |
| Inoculum (cfu) 10-100 | Growth : Good |
| Organism | Escherichia coli ATCC 25922 |
| Inoculum (cfu) 10-100 | Growth : None - poor |
| Organism | Salmonella paratyphi A ATCC 9150 |
| Inoculum (cfu) 10-100 | Growth : Good |
| Organism | Salmonella paratyphi B ATCC 8759 |
| Inoculum (cfu) 10-100 | Growth : Good |
| Organism | Salmonella typhimurium ATCC 14028 |
| Inoculum (cfu) 10-100 | Growth : Good |
| Organism | Shigella flexneri ATCC 12022 |
| Inoculum | Growth : Inhibited |
Applications :
Used for selective enrichment for the isolation of Salmonella, when used with MKTT Novobiocin supplement.
Composition :
| Ingredients | gm/lt. |
| Meat extract | 4.30 |
| Casein enzymic hydrolysate | 8.60 |
| Ox bile | 4.78 |
| Sodium chloride | 2.60 |
| Calcium carbonate | 38.70 |
| Sodium thiosulphate, anhydrous | 30.50 |
| Brilliant Green | 0.0095 |
Directions :
- Add 89.5gm of MKTTn in 1 .0 liter of distilled water/deionized water.
2. Gently heat and bring to boiling.
3. Do not Autoclave.
4. Cool to 45 - 50°C. Immediately before use, aseptically add 20 ml of iodine solution (20 grams iodine and 25 grams potassium iodide in 100 ml sterile distilled water) along with rehydrated contents of 1 vial of Novobiocin MKTT Supplement (31601).
5. Mix well to disperse calcium carbonate uniformly before dispensing in sterile tubes.
| Product Code | 76135-500 Gms |
|---|---|
| HSN Code | 38210000 |
| Brand | SRL |
| Usually dispatched in | 7-10 DAYS |
| Pack Size | 500 Gms |
DESCRIPTION
Storage: 8 to 25°C (Cool & dry area)
Shelf life: 36 Months
Specifications
| Appearance (Colour) | Pale green |
| Appearance (Form) | Free flowing, homogeneous powder |
| Solubility | 89.50 gm/liter |
| Solubility before autoclaving (Clarity) | Opaque suspension which on standing gives a heavy white precipitate |
| pH (25°C) | 8.2 ± 0.2 |
| Prepared Medium Appearance after autoclaving (Clarity) | Opaque suspension which on standing gives a heavy white precipitate |
| Prepared Medium Appearance after autoclaving (Colour) | Pale green |
| Cultural Response | Inoculate and incubate at 43°C for 18-48 hours, with added 20ml iodine solution and MKTT Novobiocin Supplement (31601), when subcultured on Soyabean Casein Digest Agar (33291) |
| Organism | Proteus vulgaris ATCC 13315 |
| Inoculum (cfu) 10-100 | Growth : None - poor |
| Organism | Salmonella enteritidis ATCC 13076 |
| Inoculum (cfu) 10-100 | Growth : Good |
| Organism | Escherichia coli ATCC 25922 |
| Inoculum (cfu) 10-100 | Growth : None - poor |
| Organism | Salmonella paratyphi A ATCC 9150 |
| Inoculum (cfu) 10-100 | Growth : Good |
| Organism | Salmonella paratyphi B ATCC 8759 |
| Inoculum (cfu) 10-100 | Growth : Good |
| Organism | Salmonella typhimurium ATCC 14028 |
| Inoculum (cfu) 10-100 | Growth : Good |
| Organism | Shigella flexneri ATCC 12022 |
| Inoculum | Growth : Inhibited |
Applications :
Used for selective enrichment for the isolation of Salmonella, when used with MKTT Novobiocin supplement.
Composition :
| Ingredients | gm/lt. |
| Meat extract | 4.30 |
| Casein enzymic hydrolysate | 8.60 |
| Ox bile | 4.78 |
| Sodium chloride | 2.60 |
| Calcium carbonate | 38.70 |
| Sodium thiosulphate, anhydrous | 30.50 |
| Brilliant Green | 0.0095 |
Directions :
- Add 89.5gm of MKTTn in 1 .0 liter of distilled water/deionized water.
2. Gently heat and bring to boiling.
3. Do not Autoclave.
4. Cool to 45 - 50°C. Immediately before use, aseptically add 20 ml of iodine solution (20 grams iodine and 25 grams potassium iodide in 100 ml sterile distilled water) along with rehydrated contents of 1 vial of Novobiocin MKTT Supplement (31601).
5. Mix well to disperse calcium carbonate uniformly before dispensing in sterile tubes.