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M‐MLV (Recombinant)

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₹16,000.00 + GST 5% off
₹15,200.00 + ₹1,824.00 GST
₹17,024.00 (Incl. of all taxes)
In stock
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Overview
Specifications
Appearance (Form) Liquid
Appearance (Colour) Colourless
Appearance (Clarity) Clear
Absence of nucleases Tested using relevant procedures
Amplification capability Tested by RT-PCR
Notes
  Description:
Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) uses single-stranded RNA or DNA in the presence of a primer to synthesize a complementary DNA
strand.

This enzyme is isolated from E. coli expressing a portion of the pol gene of M-MLV on a plasmid. The enzyme is used to synthesize first-strand cDNA up to 5 kb.

The enzyme has increased thermal stability, that allows the reaction to be carried out at a higher temperature (optimum activity at 50°C). It increases the efficiency and specificity of those transcribed RNA regions which are rich in GC pairs and/or contain secondary structures. The enzyme has no 3’ to 5’ exonuclease and reduced RNaseH activity, that improves the synthesis of a full-length cDNA, even from long mRNA templates, using random priming. The enzyme gives high yields of first strand cDNa synthesis up to 10kb long.

Applications:
PCR,
real-time PCR
second strand cDNA synthesis
DNA labeling
Analysis of RNA by primer extension

Includes:

10000U
· M-MLV (Recombinant) (200U/μl ) - 50 μl
· Reaction Buffer (10X) - 100 μl
More Information
Product Code 76516-10000 Units
HSN Code 38229090
Brand SRL
Usually dispatched in 2-3WEEK
Pack Size 10000 Units
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  1. M‐MLV (Recombinant)
    M‐MLV (Recombinant)
    ₹16,000.00 + GST 5% off
    ₹15,200.00 + ₹1,824.00 GST
    ₹17,024.00 (Incl. of all taxes)
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Details
Specifications
Appearance (Form) Liquid
Appearance (Colour) Colourless
Appearance (Clarity) Clear
Absence of nucleases Tested using relevant procedures
Amplification capability Tested by RT-PCR
Notes
  Description:
Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) uses single-stranded RNA or DNA in the presence of a primer to synthesize a complementary DNA
strand.

This enzyme is isolated from E. coli expressing a portion of the pol gene of M-MLV on a plasmid. The enzyme is used to synthesize first-strand cDNA up to 5 kb.

The enzyme has increased thermal stability, that allows the reaction to be carried out at a higher temperature (optimum activity at 50°C). It increases the efficiency and specificity of those transcribed RNA regions which are rich in GC pairs and/or contain secondary structures. The enzyme has no 3’ to 5’ exonuclease and reduced RNaseH activity, that improves the synthesis of a full-length cDNA, even from long mRNA templates, using random priming. The enzyme gives high yields of first strand cDNa synthesis up to 10kb long.

Applications:
PCR,
real-time PCR
second strand cDNA synthesis
DNA labeling
Analysis of RNA by primer extension

Includes:

10000U
· M-MLV (Recombinant) (200U/μl ) - 50 μl
· Reaction Buffer (10X) - 100 μl
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