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Listeria Identification Agar Base (PALCAM)

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₹7,900.00 + GST 8% off
₹7,268.00 + ₹1,308.24 GST
₹8,576.24 (Incl. of all taxes)
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Overview

DESCRIPTION

Concentration 68.80 gm/lit.
Storage : 8 to 25°C (Cool & dry area)
Shelf Life : 36 Months

Specifications
Appearance (Colour) Biege to pink
Appearance (Form) Free flowing, homogeneous powder
Solubility 68.80 gm/liter
pH (25°C) 7.0 ± 0.2
Solubility before autoclaving (Clarity) Clear to very slightly opalescent
Gel strength Firm, comparable with 1.5% agar gel
Prepared Medium Appearance after autoclaving (Clarity) Clear to very slightly opalescent
Prepared Medium Appearance after autoclaving (Colour) Red
Cultural Response Inoculate and incubate at 37 ± 2°C for 24-48 hours
Organism Listeria monocytogenes ATCC 19112
Inoculum (cfu) 10-100 Growth : Good
Recovery rate : >=50.0%
Colony characteristics : Grey green with black centre and black halo
Organism Listeria monocytogenes ATCC 19117
Inoculum (cfu) 10-100 Growth : Good
Recovery rate : >=50.0%
Colony characteristics : Grey green with black centre and black halo
Organism Staphylococcus aureus ATCC 25923
Inoculum (cfu) 10-100 Growth : None-poor
Recovery rate : <=10.0%
Colony characteristics : Yellow colonies with yellow halo
Organism Enterococcus faecalis ATCC 29212
Inoculum (cfu) 10-100 Growth : None-poor
Recovery rate : <=10.0%
Colony characteristics : Grey colonies with brown green halo
Notes
  Applications:
Used for isolation of Listeria sp. from clinical and non-clinical samples.
Composition:
Ingredient : gm/lt.
Peptic digest of animal tissue : 23.00
Starch : 1.00
Sodium chloride : 5.00
Mannitol : 10.00
Esculin : 0.80
Dextrose : 0.50
Ammonium ferric citrate : 0.50
Lithium chloride : 15.00
Phenol Red : 0.08
Agar : 13.00
Directions:
1. Add 34.40 gm powder to 500 ml distilled/deionized water.
2. Gently heat and bring to boil.
3. Autoclave at 15 psi at 121° C for 15 minutes.
4. Cool to 45-50°C and aseptically add rehydrated contents of 1 vial of Listeria Selective Supplement (PALCAM) (90345).
5. Mix well.
More Information
Product Code 96310-500 Gms
HSN Code 38210000
Brand SRL
Usually dispatched in 7-10 DAYS
Pack Size 500 Gms
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  1. Listeria Identification Agar Base (PALCAM)
    Listeria Identification Agar Base (PALCAM)
    ₹7,900.00 + GST 8% off
    ₹7,268.00 + ₹1,308.24 GST
    ₹8,576.24 (Incl. of all taxes)
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Details

DESCRIPTION

Concentration 68.80 gm/lit.
Storage : 8 to 25°C (Cool & dry area)
Shelf Life : 36 Months

Specifications
Appearance (Colour) Biege to pink
Appearance (Form) Free flowing, homogeneous powder
Solubility 68.80 gm/liter
pH (25°C) 7.0 ± 0.2
Solubility before autoclaving (Clarity) Clear to very slightly opalescent
Gel strength Firm, comparable with 1.5% agar gel
Prepared Medium Appearance after autoclaving (Clarity) Clear to very slightly opalescent
Prepared Medium Appearance after autoclaving (Colour) Red
Cultural Response Inoculate and incubate at 37 ± 2°C for 24-48 hours
Organism Listeria monocytogenes ATCC 19112
Inoculum (cfu) 10-100 Growth : Good
Recovery rate : >=50.0%
Colony characteristics : Grey green with black centre and black halo
Organism Listeria monocytogenes ATCC 19117
Inoculum (cfu) 10-100 Growth : Good
Recovery rate : >=50.0%
Colony characteristics : Grey green with black centre and black halo
Organism Staphylococcus aureus ATCC 25923
Inoculum (cfu) 10-100 Growth : None-poor
Recovery rate : <=10.0%
Colony characteristics : Yellow colonies with yellow halo
Organism Enterococcus faecalis ATCC 29212
Inoculum (cfu) 10-100 Growth : None-poor
Recovery rate : <=10.0%
Colony characteristics : Grey colonies with brown green halo
Notes
  Applications:
Used for isolation of Listeria sp. from clinical and non-clinical samples.
Composition:
Ingredient : gm/lt.
Peptic digest of animal tissue : 23.00
Starch : 1.00
Sodium chloride : 5.00
Mannitol : 10.00
Esculin : 0.80
Dextrose : 0.50
Ammonium ferric citrate : 0.50
Lithium chloride : 15.00
Phenol Red : 0.08
Agar : 13.00
Directions:
1. Add 34.40 gm powder to 500 ml distilled/deionized water.
2. Gently heat and bring to boil.
3. Autoclave at 15 psi at 121° C for 15 minutes.
4. Cool to 45-50°C and aseptically add rehydrated contents of 1 vial of Listeria Selective Supplement (PALCAM) (90345).
5. Mix well.
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