Hydrophobic Chromatography Teaching Kit, 5 expts
₹8,649.00 + ₹1,037.88 GST
₹9,686.88 (Incl. of all taxes)
DESCRIPTION:
Hydrophobic Chromatography kit is designed to teach students the basic principle of hydrophobic interaction chromatography (HIC).Green Fluorescent Protein (GFP), a hydrophobic protein is utilized as a sample for purification. In the kit, bacterial lysate is provided as a sample. GFP is purified using HIC technique. GFP has several stretches of hydrophobic amino acids, which results in the total protein being highly hydrophobic. When the supernatant, rich in GFP, is passed over a HIC column in a high salt buffer (Binding Buffer) the hydrophobic regions of the GFP stick to the HIC beads. Other proteins that are less hydrophobic (or more hydrophilic) pass right through the column. This single procedure allows the purification of GFP from a complex mixture of bacterial proteins. The GFP is eluted with low salt elution buffer. The low salt buffer will disrupt the hydrophobic interactions between the GFP and column bed, enabling GFP to let go and “elute” from the column.
The kit contents are sufficient for 5 experiments.
MATERIALS PROVIDED:
• Column Matrix
• Binding Buffer
• Equilibration Buffer
• Wash Buffer
• Elution Buffer
• Sample
• Storage Buffer
• Instruction Manual
| Product Code | 6120200011730 - KT202 |
|---|---|
| HSN Code | 38220090 |
| Brand | GeNei |
| Usually dispatched in | 1-2 weeks |
| Pack Size | 1 EA |
DESCRIPTION:
Hydrophobic Chromatography kit is designed to teach students the basic principle of hydrophobic interaction chromatography (HIC).Green Fluorescent Protein (GFP), a hydrophobic protein is utilized as a sample for purification. In the kit, bacterial lysate is provided as a sample. GFP is purified using HIC technique. GFP has several stretches of hydrophobic amino acids, which results in the total protein being highly hydrophobic. When the supernatant, rich in GFP, is passed over a HIC column in a high salt buffer (Binding Buffer) the hydrophobic regions of the GFP stick to the HIC beads. Other proteins that are less hydrophobic (or more hydrophilic) pass right through the column. This single procedure allows the purification of GFP from a complex mixture of bacterial proteins. The GFP is eluted with low salt elution buffer. The low salt buffer will disrupt the hydrophobic interactions between the GFP and column bed, enabling GFP to let go and “elute” from the column.
The kit contents are sufficient for 5 experiments.
MATERIALS PROVIDED:
• Column Matrix
• Binding Buffer
• Equilibration Buffer
• Wash Buffer
• Elution Buffer
• Sample
• Storage Buffer
• Instruction Manual