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Hugh Leifson Medium (B/S)

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₹1,100.00 + GST 10% off
₹990.00 + ₹178.20 GST
₹1,168.20 (Incl. of all taxes)
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Overview

DESCRIPTION

Storage : 8 to 25°C (Cool & dry area)
Shelf Life : 36 Months

Specifications
Appearance (Clarity) Light yellow with bluish green tint
Appearance (Colour) Free flowing, homogeneous powder
Solubility 20.33 gm/liter
Solubility before autoclaving (Clarity) Clear to very slightly opalescent
pH (25°C) 7.1 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity) Clear to very slightly opalescent
Prepared Medium Appearance after autoclaving (Colour) Greenish blue
Cultural Response Inoculate and incubate at 37° C for 18 - 48 hours
Organism Enterobacter aerogenes ATCC 13048
Inoculum (cfu) 10-100 Growth:Good
Motility : Positive
Aerobic Fermentation : Acid(yellow), Gas production : Positive
Anaerobic Fermentation : Acid(yellow), Gas production : Positive
Organism Escherichia coli ATCC 25922
Inoculum (cfu) 10-100 Growth : Good
Motility : Positive
Aerobic Fermentation : Acid(yellow), Gas production : Positive
Anaerobic Fermentation : Acid(yellow), Gas production : Positive
Organism Pseudomonas aeruginosa ATCC 27853
Inoculum (cfu) 10-100 Growth:Good
Motility : Positive
Aerobic Fermentation : Acid(yellow), Gas production : Positive
Anaerobic Fermentation : Green/Blue, Gas production : Negative
Organism Salmonella typhimurium ATCC 6539
Inoculum (cfu) 10-100 Growth:Good
Motility : Positive
Aerobic Fermentation : Acid(yellow), Gas production : Positive
Anaerobic Fermentation : Acid(yellow), Gas production : Positive
Notes
  Applications:
Used to distinguish between anaerobic and aerobic breakdown of carbohydrate (Dextrose).

Composition:
Ingredients : gm/lt.
Peptone : 2.00
Sodium chloride : 5.00
Dipotassium phosphate : 0.30
Dextrose : 10.0
Bromothymol Blue : 0.03
Agar : 3.00

Directions:
1. Add 20.33 gm powder to 1.0 liter of distilled/deionized water and mix thoroughly.
2. Gently heat and bring to boiling
3. Autoclave at 15 psi pressure at 121°C for 15 minutes.
More Information
Product Code 26221-100 Gms
HSN Code 38210000
Brand SRL
Usually dispatched in 7-10 DAYS
Pack Size 100 Gms
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  1. Hugh Leifson Medium (B/S)
    Hugh Leifson Medium (B/S)
    ₹1,100.00 + GST 10% off
    ₹990.00 + ₹178.20 GST
    ₹1,168.20 (Incl. of all taxes)
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Details

DESCRIPTION

Storage : 8 to 25°C (Cool & dry area)
Shelf Life : 36 Months

Specifications
Appearance (Clarity) Light yellow with bluish green tint
Appearance (Colour) Free flowing, homogeneous powder
Solubility 20.33 gm/liter
Solubility before autoclaving (Clarity) Clear to very slightly opalescent
pH (25°C) 7.1 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity) Clear to very slightly opalescent
Prepared Medium Appearance after autoclaving (Colour) Greenish blue
Cultural Response Inoculate and incubate at 37° C for 18 - 48 hours
Organism Enterobacter aerogenes ATCC 13048
Inoculum (cfu) 10-100 Growth:Good
Motility : Positive
Aerobic Fermentation : Acid(yellow), Gas production : Positive
Anaerobic Fermentation : Acid(yellow), Gas production : Positive
Organism Escherichia coli ATCC 25922
Inoculum (cfu) 10-100 Growth : Good
Motility : Positive
Aerobic Fermentation : Acid(yellow), Gas production : Positive
Anaerobic Fermentation : Acid(yellow), Gas production : Positive
Organism Pseudomonas aeruginosa ATCC 27853
Inoculum (cfu) 10-100 Growth:Good
Motility : Positive
Aerobic Fermentation : Acid(yellow), Gas production : Positive
Anaerobic Fermentation : Green/Blue, Gas production : Negative
Organism Salmonella typhimurium ATCC 6539
Inoculum (cfu) 10-100 Growth:Good
Motility : Positive
Aerobic Fermentation : Acid(yellow), Gas production : Positive
Anaerobic Fermentation : Acid(yellow), Gas production : Positive
Notes
  Applications:
Used to distinguish between anaerobic and aerobic breakdown of carbohydrate (Dextrose).

Composition:
Ingredients : gm/lt.
Peptone : 2.00
Sodium chloride : 5.00
Dipotassium phosphate : 0.30
Dextrose : 10.0
Bromothymol Blue : 0.03
Agar : 3.00

Directions:
1. Add 20.33 gm powder to 1.0 liter of distilled/deionized water and mix thoroughly.
2. Gently heat and bring to boiling
3. Autoclave at 15 psi pressure at 121°C for 15 minutes.
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