HEX 50 nm

DESCRIPTION:

Synthesis of a gene “synthetically” allows scientists to avoid difficult gene cloning experiments where:

• Gene expression is tissue specific, enzyme treatment specific and / or growth stage specific. Thus, RT-PCR based gene cloning experiments would be very tedious.
• Exon – intron boundaries not well demarcated because of nonavailability of genome sequence data and or complete gene sequence data (showing intron – exon boundaries).
• Synthesis of DNA fragments containing regulatory sequences is required.

Features:

• The synthetic gene would be synthesized following “modified recursive PCR” technology
• The gene will be cloned in a standard cloning vector (pUC18).
• Multiple clones will be sequenced and the clone having no or minimum mutations will be selected.
• Mutations, if present, would be reverted ‘free of cost’.
• A confirmed clone having no mutations would be delivered to the scientist.

Deliverables:
1. Synthetic gene: synthesized, cloned and purified DNA – 5 μg
2. Original sequencing data
3. Blasted sequence data (showing 100% homology)
4. Project Report