Genomic DNA Extraction (from leaves) Kit (Teaching)
Special Price
₹11,088.00
(Incl. of all taxes)
GST
₹12,320.00
₹11,000.00 + GST
10% off
₹9,900.00
+ ₹1,188.00 GST
₹11,088.00 (Incl. of all taxes)
Overview
| Specifications |
| Agarose gel electrophoresis | Extracted DNAs molecular size is compared with High range DNA marker | | A260/A280 | 1.6-1.9 | | DNase activity | None detected | | Stability | Stable for one year | |
| |
| | Description:
The overall goal is to isolate chromosomal DNA from leaves. The extraction buffer breaks the cell walls to release the cellular constituents. The buffer contains a detergent cetyl methylammonium bromide (CTAB) that disrupts the cellular membranes to release the DNA, EDTA which minimizes nuclease activity and NaCl that removes polysaccharide. The contaminating RNA is removed by digestion with RNase A. Finally, nucleic acids are precipitated in water-alcohol mixture.
Includes:
Extraction buffer - 25 ml
β Mercaptoethanol - 50 µl
Chloroform - 50 ml
Isoamylalcohol - 2 ml
DNA precipitation solution-50 ml
Wash solution -50 ml
TE Buffer - 2 ml
RNase A (10 mg/ml) – 50 µl
6X gel loading dye -100 µl
Agarose -500 mg
Ethidium bromide -30 µl
50X TAE Buffer -25 ml
DNA marker -60 µl | |
More Information
| Product Code |
61498-15 expt. Kit |
| HSN Code |
38229090 |
| Brand |
SRL |
| Usually dispatched in |
2-3WEEK |
| Pack Size |
15 expt. Kit |
