GeNei™ One Step M-MuLV RT-PCR Kit, 50 reactions

One Step RT PCR Kit provides specific, sensitive and easy to use system for the detection and analysis of RNA. By using this convenient one step formulation, one can perform both DNA synthesis and PCR amplification in a single tube, using gene specific primers and target RNAs from either total RNA or mRNA. The system uses a mixture of Reverse transcriptase, Hotstart Taq DNA Polymerase and a high fidelity enzyme in an optimized (single) reaction buffer.

Applications:

• Analysis of Gene expression
• Detection of rare and viral RNA
• Characterization of RNA specific variants

Highlights:

• Hotstart format for higher sensitivity, specificity and yield.
• Yields product with high fidelity for cloning and sequencing.
• Fast and easy screening of gene expression.
• Lower risk of sample cross contamination aids in simultaneous analysis of multiple samples.
• Reduced reaction variability.
• Less hands on time.

Materials provided:

• RT-PCR Enzyme mix
• 2X RT-PCR reaction mixture
• RNasin
• Water (Protease, DNase and RNase free)
• Instruction Manual