GeNei™ Instant Cloning Kit, 10 reactions
₹7,905.00 + ₹948.60 GST
₹8,853.60 (Incl. of all taxes)
Description
PCR products amplified with Taq DNA Polymerase carry an additional single 3′-A (in most cases) that poses problem in cloning directly into vector. T – vector with single 3′-T extension at the site of insertion is a simple solution. Since, the ligation involves single base overhang joining, which is more difficult than blunt end joining. INSTANT Cloning Kit contains Instant T4 DNA ligase and 2X Instant ligation buffer to enhance the process
Highlights :
•One hour ligation at room temperature (25°C).
•Amplified product can be ligated directly or after purification to the vector.
•No enzymatic manipulation of product required.
•Blue-white selection marker makes screening easier.
•Insert can be released using Nco I enzyme.
•Sequencing of insert can be done using SP6 and T7 promoter primers.
•30-60% clones expected depending on the insert size.
Storage : -20°C
| Product Code | 1660630011730-KT63A |
|---|---|
| HSN Code | 38220090 |
| Brand | GeNei |
| Usually dispatched in | 2-3 WEEKS |
| Pack Size | 1 EA |
Description
PCR products amplified with Taq DNA Polymerase carry an additional single 3′-A (in most cases) that poses problem in cloning directly into vector. T – vector with single 3′-T extension at the site of insertion is a simple solution. Since, the ligation involves single base overhang joining, which is more difficult than blunt end joining. INSTANT Cloning Kit contains Instant T4 DNA ligase and 2X Instant ligation buffer to enhance the process
Highlights :
•One hour ligation at room temperature (25°C).
•Amplified product can be ligated directly or after purification to the vector.
•No enzymatic manipulation of product required.
•Blue-white selection marker makes screening easier.
•Insert can be released using Nco I enzyme.
•Sequencing of insert can be done using SP6 and T7 promoter primers.
•30-60% clones expected depending on the insert size.
Storage : -20°C