GeNei™ HotStart Blue Dye PCR mastermix (2X)

₹5,940.00 + GST 10% off
₹5,346.00 + ₹962.28 GST
₹6,308.28 (Incl. of all taxes)
In stock
Overview

Description The HotStart PCR Master Mix (2X)is a premixed, ready to use solution containing HotStart Taq DNA Polymerase, dNTPs, standard reaction buffer, stabilizers and an easy to track blue loading dye. Inclusion of tracking dye allows direct loading & analysis of PCR products by agarose gel electrophoresis following PCR amplification. The Ready to use PCR mix provides robust, reliable & consistent performance for PCR applications where specificity & sensitivity are desired. The HotStart PCR Master Mix is supplied at 2X concentration to accommodate the addition of Magnesium chloride, additives like BSA, DMSO, glycerol etc.

Storage:-20°C.

Performance Test: The HotStart PCR Master Mix (2X) is tested extensively for its reproducible performance in critical PCR amplifications. Mix is tested for amplification of 131 bp fragment of TNF gene and 400 bp fragment of β-Globin gene from human genomic DNA using non-optimal primers. Mix is tested for detecting different subtypes of Human Papilloma virus(HPV) using consensus primers.

Highlights: • Saves time-directly from PCR to gel electrophoresis. • Room temperature assembly. • H igher sensitivity & specificity compared to conventional Taq DNA Polymerase. • Efficient amplification for as low as 25μl reaction volume.

More Information
Product Code 0604400021730-MME44
HSN Code 35079099
Brand GeNei
Usually dispatched in 2-3 WEEKS
Pack Size 1X100 rxns

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Description The HotStart PCR Master Mix (2X)is a premixed, ready to use solution containing HotStart Taq DNA Polymerase, dNTPs, standard reaction buffer, stabilizers and an easy to track blue loading dye. Inclusion of tracking dye allows direct loading & analysis of PCR products by agarose gel electrophoresis following PCR amplification. The Ready to use PCR mix provides robust, reliable & consistent performance for PCR applications where specificity & sensitivity are desired. The HotStart PCR Master Mix is supplied at 2X concentration to accommodate the addition of Magnesium chloride, additives like BSA, DMSO, glycerol etc.

Storage:-20°C.

Performance Test: The HotStart PCR Master Mix (2X) is tested extensively for its reproducible performance in critical PCR amplifications. Mix is tested for amplification of 131 bp fragment of TNF gene and 400 bp fragment of β-Globin gene from human genomic DNA using non-optimal primers. Mix is tested for detecting different subtypes of Human Papilloma virus(HPV) using consensus primers.

Highlights: • Saves time-directly from PCR to gel electrophoresis. • Room temperature assembly. • H igher sensitivity & specificity compared to conventional Taq DNA Polymerase. • Efficient amplification for as low as 25μl reaction volume.

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