E.coli JM109, 1 vial
₹5,865.00 + ₹1,055.70 GST
₹6,920.70 (Incl. of all taxes)
Description
JM109 is supplied in lyophilized form.
Features & Applications :
1. JM109 cells carry the F’ episome for pili allowing infection by filamentous phage such as M13 and by M13 helper phage for single stranded rescue from phagemid vectors.
2. F’ episome also has the lacIq repressor gene producing10 fold more lac repressor than is found in most strains thereby ensuring more stringent repression of toxic proteins.
3. F’ episome has the lacIq????m15 mutation which provides the complementation of the β-galactosidase gene allowing blue/white selection assay.
4. Insert stability is ensured due to Rec A– status. This strain also lacks the E.coli K restriction system thus preventing restriction of cloned DNA.
5. End A1 mutation ensures quality and improved yield of plasmid DNA.
6. Allows cloning with cleavage of transformed DNA by endogenous restriction endonucleases. DNA prepared from these strains can be used to transform rk E.coli. strains.
Genotype: end A1, rec A1, gyr A96, thi, hsd R17 (rk , mk ), rel A1, supE44, ???? (lac – pro AB), [F’, tra D36, proAB, lac IqZ????M15].
| Product Code | 5101600011730-EC6 |
|---|---|
| HSN Code | 29349900 |
| Brand | GeNei |
| Usually dispatched in | 2-3 WEEKS |
| Pack Size | 1 EA |
Description
JM109 is supplied in lyophilized form.
Features & Applications :
1. JM109 cells carry the F’ episome for pili allowing infection by filamentous phage such as M13 and by M13 helper phage for single stranded rescue from phagemid vectors.
2. F’ episome also has the lacIq repressor gene producing10 fold more lac repressor than is found in most strains thereby ensuring more stringent repression of toxic proteins.
3. F’ episome has the lacIq????m15 mutation which provides the complementation of the β-galactosidase gene allowing blue/white selection assay.
4. Insert stability is ensured due to Rec A– status. This strain also lacks the E.coli K restriction system thus preventing restriction of cloned DNA.
5. End A1 mutation ensures quality and improved yield of plasmid DNA.
6. Allows cloning with cleavage of transformed DNA by endogenous restriction endonucleases. DNA prepared from these strains can be used to transform rk E.coli. strains.
Genotype: end A1, rec A1, gyr A96, thi, hsd R17 (rk , mk ), rel A1, supE44, ???? (lac – pro AB), [F’, tra D36, proAB, lac IqZ????M15].