DH5a Competent Cells for Subcloning
Special Price
₹2,798.32
(Incl. of all taxes)
GST
₹2,945.60
₹2,630.00 + GST
5% off
₹2,498.50
+ ₹299.82 GST
₹2,798.32 (Incl. of all taxes)
Overview
| Specifications |
| Antibiotic Sensitivity (Positive) | DH5α Competent cells, 10μl were streaked into a LB Agar plate containing Nalidixic acid, will form colonies after incubation for overnight at 37°C. | | Antibiotic Sensitivity (Negative) | DH5α Competent cells, 10μl were streaked into a LB Agar plate containing Ampicillin, will not form colonies after incubation for overnight at 37°C. | | Blue - White Screening | DH5α Competent cells were shown to be suitable for blue white screening by α - complementation of the β - galactosidase gene using pUC19. | | Transformation Efficiency | DH5α Competent cells, 50 μl were transformed with 200pg of pUC19 DNA using standard protocol. Incubation overnight on LB Agar plates shown >=1x 106 cfu/μg of DNA. | |
| |
| | Description:
DH5α Competent Cells are ideal cells that are made efficient chemically for transformation techniques. They are recommended for routine subcloning into plasmid vectors giving a transformation efficiency of ≥1 × 10⁶cfu/μg. Subcloning efficiency cells are not suitable for the generation of cDNA libraries. The φ80dlacZΔM15 marker provides α-complementation of the β-galactosidase gene from pUC or similar vectors to allow blue/white colony screening on bacterial agar plates containing X-Gal and similar chemicals.
Includes:
2x 0.1ml
DH5α Competent cells - 2 X 0.1ml
pUC19 (10ng/μl) - 50 μl | |
More Information
| Product Code |
64438-2 x0.1 ml |
| HSN Code |
38229090 |
| Brand |
SRL |
| Usually dispatched in |
2-3WEEK |
| Pack Size |
2 x0.1 ml |
