Decarboxylase Test Medium Base (Falkow)
Special Price
₹1,194.16
(Incl. of all taxes)
GST
₹1,298.00
₹1,100.00 + GST
8% off
₹1,012.00
+ ₹182.16 GST
₹1,194.16 (Incl. of all taxes)
Overview
DESCRIPTION
(Decarboxylase Basal Medium, Decarboxylase Media, Falkow)
Concentration 9.00 gm/lit.
Storage : 8 to 25°C (Cool & dry area)
Shelf Life : 36 Months
| Specifications |
| Appearance (Colour) | Yellow | | Appearance (Form) | Free flowing, homogeneous powder | | Solubility | 9.00 gm/liter | | Solubility before autoclaving (Clarity) | Clear without any precipitate | | pH (25°C) | 6.8 ± 0.2 | | Prepared Medium Appearance after autoclaving (Clarity) | Clear without any precipitate | | Prepared Medium Appearance after autoclaving (Colour) | Purple | | Cultural Response | Inoculate and incubate at 37°C for 4 days | | Organism | Escherichia coli ATCC 25922 | | Inoculum (cfu) 10-100 | Growth : Good
Lysine : (±)ve
Arginine : (±)ve
Ornithine : (±)ve | | Organism | Enterobacter aerogenes ATCC 13048 | | Inoculum (cfu) 10-100 | Growth : Good
Lysine : (+)ve
Arginine : (-)ve
Ornithine : (+)ve | | Organism | Salmonella serotype Typhi ATCC 6539 | | Inoculum (cfu) 10-100 | Growth : Good
Lysine : (+) ve
Arginine : (+)ve or (-)ve
Ornithine : (+)ve | | Organism | Klebsiella pneumoniae ATCC 13883 | | Inoculum (cfu) 10-100 | Growth : Good
Lysine : (+)ve
Arginine : (-)ve
Ornithine : (-)ve | | Organism | Pseudomonas aeruginosa ATCC 27853 | | Inoculum (cfu) 10-100 | Growth : Good
Lysine : (-)ve
Arginine : (+)ve
Ornithine :(-)ve | | Organism | Shigella flexneri ATCC 12022 | | Inoculum (cfu) 10-100 | Growth : Good
Lysine :( -)ve
Arginine : (+)ve or (-)ve
Ornithine : (-)ve | | Key | (±)ve : variable reaction
(+)ve : positive reaction,purple colour
(-)ve : negative reaction,yellow colour | |
| |
| | Applications:
For the differentiation of bacteria based on their ability to decarboxylate the amino acid.
Composition:
Ingredients : gm/lt.
Peptone : 5.00
Yeast extract : 3.00
Dextrose : 1.00
Bromocresol Purple : 0.02
Directions:
1. Add 9.00 gm powder to 1.0 liter of distilled/deionized water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Distribute the medium into 4 equal parts into test tubes.
4. In the first test tube no amino acid is to be added.
5. To the remaining 3 parts, add separately 3 L-amino acids, L-Lysine hydrochloride, L-Arginine hydrochloride and L-Ornithine hydrochloride to a final concentration of 0.5%.
6. Dispense in 3 -4 ml quantities in screw capped tubes.
7. Autoclave at 15 psi pressure at 121°C for 15 minutes.
Note : To avoid false alkalinization at the surface of medium ;add liquid paraffin to a height of about 5mm
before sterilization. | |
More Information
| Product Code |
96640-100 Gms |
| HSN Code |
38210000 |
| Brand |
SRL |
| Usually dispatched in |
7-10 DAYS |
| Pack Size |
100 Gms |
