CLED Agar

₹3,900.00 + GST 8% off
₹3,588.00 + ₹645.84 GST
₹4,233.84 (Incl. of all taxes)
In stock
Overview

DESCRIPTION

(Brolacin Agar)
Concentration 36.14 gm/lit.
Storage : 8 to 25°C (Cool & dry area)
Shelf Life : 36 Months

Specifications
Appearance (Colour) Biege with slight green tint
Appearance (Form) Free flowing, homogeneous powder
Solubility 36.14 gm/liter
Solubility before autoclaving (Clarity) Very slightly opalescent
Gel strength Firm, comparable with 1.5% agar gel
pH (25°C) 7.3 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity) Very slightly opalescent
Prepared Medium Appearance after autoclaving (Colour) Green
Cultural Response Inoculate and incubate at 37°C for 18 - 24 hours
Organism Escherichia coli ATCC 25922
Inoculum (cfu) 10-100 Growth : Good, Recovery Rate : >=70%,
Colony colour : Yellow
Organism Salmonella typhi ATCC 6539
Inoculum (cfu) 10-100 Growth : Good, Recovery Rate : >=70%,
Colony colour : Blue
Organism Klebsiella pneumoniae ATCC 13883
Inoculum (cfu) 10-100 Growth : Good, Recovery Rate : >=70%,
Colony colour : Yellow to whitish blue
Organism Proteus vulgaris ATCC 13315
Inoculum (cfu) 10-100 Growth: Good, swarming inhibited, Recovery Rate:>=70%,
Colony colour : Blue to bluish green
Organism Staphylococcus aureus ATCC 25923
Inoculum (cfu) 10-100 Growth : Good, Recovery Rate : >=70%,
Colony colour : Yellow
Organism Enterococcus faecalis ATCC 29212
Inoculum (cfu) 10-100 Growth : Good, Recovery Rate : >=70%,
Colony colour : Slight yellowish to greenish
Notes
  Applications:
For the isolation, enumeration and presumptive identification of microorganisms from urine.
Composition:
Ingredients : gm/lt.
Peptone : 4.00
Agar : 15.00
Lactose : 10.00
Tryptone : 4.00
Beef extract (ex.buffalo) : 3.00
L-Cystine : 0.128
Bromothymol Blue : 0.02
Directions:
1. Add 36.14 gm powder to 1.0 liter of distilled/deionized water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Autoclave at 15 psi pressure at 121°C for 15 minutes.
More Information
Product Code 55769-500 Gms
HSN Code 38210000
Brand SRL
Usually dispatched in 7-10 DAYS
Pack Size 500 Gms
Purity % Escherichia coli ATCC 25922, Salmonella typhi ATCC 6539, Klebsiella pneumoniae ATCC 13883, Proteus vulgaris ATCC 13315, Staphylococcus aureus ATCC 25923, Enterococcus faecalis ATCC 29212

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DESCRIPTION

(Brolacin Agar)
Concentration 36.14 gm/lit.
Storage : 8 to 25°C (Cool & dry area)
Shelf Life : 36 Months

Specifications
Appearance (Colour) Biege with slight green tint
Appearance (Form) Free flowing, homogeneous powder
Solubility 36.14 gm/liter
Solubility before autoclaving (Clarity) Very slightly opalescent
Gel strength Firm, comparable with 1.5% agar gel
pH (25°C) 7.3 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity) Very slightly opalescent
Prepared Medium Appearance after autoclaving (Colour) Green
Cultural Response Inoculate and incubate at 37°C for 18 - 24 hours
Organism Escherichia coli ATCC 25922
Inoculum (cfu) 10-100 Growth : Good, Recovery Rate : >=70%,
Colony colour : Yellow
Organism Salmonella typhi ATCC 6539
Inoculum (cfu) 10-100 Growth : Good, Recovery Rate : >=70%,
Colony colour : Blue
Organism Klebsiella pneumoniae ATCC 13883
Inoculum (cfu) 10-100 Growth : Good, Recovery Rate : >=70%,
Colony colour : Yellow to whitish blue
Organism Proteus vulgaris ATCC 13315
Inoculum (cfu) 10-100 Growth: Good, swarming inhibited, Recovery Rate:>=70%,
Colony colour : Blue to bluish green
Organism Staphylococcus aureus ATCC 25923
Inoculum (cfu) 10-100 Growth : Good, Recovery Rate : >=70%,
Colony colour : Yellow
Organism Enterococcus faecalis ATCC 29212
Inoculum (cfu) 10-100 Growth : Good, Recovery Rate : >=70%,
Colony colour : Slight yellowish to greenish
Notes
  Applications:
For the isolation, enumeration and presumptive identification of microorganisms from urine.
Composition:
Ingredients : gm/lt.
Peptone : 4.00
Agar : 15.00
Lactose : 10.00
Tryptone : 4.00
Beef extract (ex.buffalo) : 3.00
L-Cystine : 0.128
Bromothymol Blue : 0.02
Directions:
1. Add 36.14 gm powder to 1.0 liter of distilled/deionized water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Autoclave at 15 psi pressure at 121°C for 15 minutes.
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