Cetrimide Agar, Base

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Overview

Description:

Concentration 45.30 gm/lit.
Storage : 8 to 25°C (Cool & dry area)
Shelf Life : 36 Months

Specifications
Appearance (Colour) Light cream
Appearance (Form) Free flowing, homogeneous powder
Solubility 45.30 gm/liter
Solubility before autoclaving (Clarity) Clear to very slightly opalescent
Gel strength (1.5% gel) Firm, comparable with 1.5% agar gel
pH (25°C) 7.2 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity) Opalescent
Prepared Medium Appearance after autoclaving (Colour) Light amber
Cultural Response Inoculate and incubate at 37°C for 18-24 hours
Organism Pseudomonas aeruginosa ATCC 27853
Inoculum (cfu) 10-100 Growth : Good, Recovery Rate :>=50%
Organism Pseudomonas aeruginosa ATCC 9027
Inoculum (cfu) 10-100 Growth : Good, Recovery Rate :>=50%
Organism Staphylococcus aureus ATCC 25923
Inoculum >=103, Growth : Inhibited
Organism Escherichia coli
ATCC 25922
Inoculum >=103, Growth : Inhibited
 
Notes
  Applications:
For the selective isolation, cultivation and identification of Pseudomonas aeruginosa.

Composition:
Ingredients : gm/lt.
Peptone : 20.00
Magnesium chloride : 1.40
Potassium sulphate : 10.00
Cetrimide : 0.30
Agar : 13.60

Directions:
1. Add 10 ml of glycerol to distilled/deionized water.
2. Add 45.30 gm powder to distilled/deionized water.
3. Bring volume to 1.0 liter and mix thoroughly.
4. Gently heat and bring to boiling.
5. Autoclave at 15 psi pressure at 121°C for 15 minutes.
6. If desired,add aseptically rehydrated contents of 1 vial of Nalidixic Selective Supplement (67124) to 1.0 liter of medium.
7. Mix well and dispense.
More Information
Product Code 45470-100 Gms
HSN Code 38210000
Brand SRL
Usually dispatched in 7-10 DAYS
Pack Size 100 Gms
Purity % Pseudomos aeruginosa ATCC 27853, Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922

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Description:

Concentration 45.30 gm/lit.
Storage : 8 to 25°C (Cool & dry area)
Shelf Life : 36 Months

Specifications
Appearance (Colour) Light cream
Appearance (Form) Free flowing, homogeneous powder
Solubility 45.30 gm/liter
Solubility before autoclaving (Clarity) Clear to very slightly opalescent
Gel strength (1.5% gel) Firm, comparable with 1.5% agar gel
pH (25°C) 7.2 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity) Opalescent
Prepared Medium Appearance after autoclaving (Colour) Light amber
Cultural Response Inoculate and incubate at 37°C for 18-24 hours
Organism Pseudomonas aeruginosa ATCC 27853
Inoculum (cfu) 10-100 Growth : Good, Recovery Rate :>=50%
Organism Pseudomonas aeruginosa ATCC 9027
Inoculum (cfu) 10-100 Growth : Good, Recovery Rate :>=50%
Organism Staphylococcus aureus ATCC 25923
Inoculum >=103, Growth : Inhibited
Organism Escherichia coli
ATCC 25922
Inoculum >=103, Growth : Inhibited
 
Notes
  Applications:
For the selective isolation, cultivation and identification of Pseudomonas aeruginosa.

Composition:
Ingredients : gm/lt.
Peptone : 20.00
Magnesium chloride : 1.40
Potassium sulphate : 10.00
Cetrimide : 0.30
Agar : 13.60

Directions:
1. Add 10 ml of glycerol to distilled/deionized water.
2. Add 45.30 gm powder to distilled/deionized water.
3. Bring volume to 1.0 liter and mix thoroughly.
4. Gently heat and bring to boiling.
5. Autoclave at 15 psi pressure at 121°C for 15 minutes.
6. If desired,add aseptically rehydrated contents of 1 vial of Nalidixic Selective Supplement (67124) to 1.0 liter of medium.
7. Mix well and dispense.
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