cDNAdirect™ Kit, 20 preps
₹14,348.00 + ₹1,721.76 GST
₹16,069.76 (Incl. of all taxes)
cDNAdirect™ is an optimized kit for synthesizing first-strand cDNA directly from mammalian cells in culture without first isolating RNA. The resulting first-strand cDNA is ready to be used in cloning, RT-PCR, Real time PCR and other downstream applications. In cDNAdirect™ Kit, cells are first washed with PBS to remove cell culture medium, extracellular and intracellular material released by living, dead and lysed cells. Cells are then incubated with specially designed lysis buffer at 75oC to rupture the cells and inactivate all the endogenous RNases. Following a DNase treatment to degrade the genomic DNA the cell lysate is ready for reverse transcription and PCR by two step method.
Applications:
- RT-PCR
- Real-time PCR
- Microarray
- Differential gene expression analysis
Highlights:
- No RNA isolation or purification required.
- Kit is optimized for synthesizing cDNA from small cell samples.
- Generates high quality cDNA for use in a variety of downstream applications.
- Compatible with wide range of mammalian cell types.
- cDNA synthesized is free of genomic DNA contamination.
- Rapid and simple procedure to recover cDNA in 30 mins.
- Rare transcripts can be detected.
- Simple Protocol
- Works with embryonic stem cells/lines
- The kit provides sufficient reagents for 20 reactions.
Material provided:
- 10XPBS
- cDNAdirect™ Lysis Buffer
- DNaseI
- cDNAdirect™ RT mix
- AMV Reverse Transcriptase
- RNasin
- Random Hexamer
- Positive RNA control
- Positive RNA primer pair
- Nuclease free water
Storage: Other components at -20oC. PBS at 4oC.
| Product Code | 0667800021730-KT178S |
|---|---|
| HSN Code | 38220090 |
| Brand | GeNei |
| Usually dispatched in | 2-3 WEEKS |
| Pack Size | 1 EA |
cDNAdirect™ is an optimized kit for synthesizing first-strand cDNA directly from mammalian cells in culture without first isolating RNA. The resulting first-strand cDNA is ready to be used in cloning, RT-PCR, Real time PCR and other downstream applications. In cDNAdirect™ Kit, cells are first washed with PBS to remove cell culture medium, extracellular and intracellular material released by living, dead and lysed cells. Cells are then incubated with specially designed lysis buffer at 75oC to rupture the cells and inactivate all the endogenous RNases. Following a DNase treatment to degrade the genomic DNA the cell lysate is ready for reverse transcription and PCR by two step method.
Applications:
- RT-PCR
- Real-time PCR
- Microarray
- Differential gene expression analysis
Highlights:
- No RNA isolation or purification required.
- Kit is optimized for synthesizing cDNA from small cell samples.
- Generates high quality cDNA for use in a variety of downstream applications.
- Compatible with wide range of mammalian cell types.
- cDNA synthesized is free of genomic DNA contamination.
- Rapid and simple procedure to recover cDNA in 30 mins.
- Rare transcripts can be detected.
- Simple Protocol
- Works with embryonic stem cells/lines
- The kit provides sufficient reagents for 20 reactions.
Material provided:
- 10XPBS
- cDNAdirect™ Lysis Buffer
- DNaseI
- cDNAdirect™ RT mix
- AMV Reverse Transcriptase
- RNasin
- Random Hexamer
- Positive RNA control
- Positive RNA primer pair
- Nuclease free water
Storage: Other components at -20oC. PBS at 4oC.