Bismuth Sulphite Agar (I)

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Overview

DESCRIPTION

(Bismuth Sulfite Agar (I))
Concentration 63.40 gm/lit.
Storage : 8 to 25°C (Cool & dry area)
Shelf Life : 36 Months

Specifications
Appearance (Colour) Light beige to light green
Appearance (Form) Free flowing, homogeneous powder
Solubility 63.40 gm/liter
Solubility before autoclaving (Clarity) Opaque with a flocculent precipitate
Gel strength Firm, comparable with 1.5% agar gel
pH (25°C) 7.5 ± 0.2
Prepared medium appearance after boiling (clarity) Opaque with a flocculent precipitate
Prepared medium appearance after boiling (colour) Light grey-green to medium green
Cultural Response Inoculate and incubate at 37°C for 18 -24 hours
Organism Salmonella typhimurium ATCC 14028
Inoculum (cfu) 10-100 Growth : Good, Recovery Rate : >=50%, Colony colour : Black or greenish-grey, may have sheen
Organism Salmonella typhi ATCC 6539
Inoculum (cfu) 10-100 Growth : Good, Recovery Rate : >=50%, Colony colour : Black with metallic sheen
Organism Escherichia coli ATCC 25922
Inoculum >103, Growth : Partial Inhibition, Colony colour : Dull green or brown colonies, without metallic sheen.
Organism Enterococcus faecalis ATCC 29212
Inoculum >103, Growth : Inhibited
Organism Salmonella enteritidis ATCC 13076
Inoculum (cfu) 10-100 Growth : Good, Recovery Rate : >=50%, Colony colour : Black with metallic sheen
Notes
  Applications :
For the selective isolation and identification of Salmonella typhi and other enteric bacilli.
Composition:
Ingredients : gm/lt.
Peptone : 10.00
Agar : 24.00
D- Glucose monohydrate : 5.00
Ferric citrate : 0.40
Brilliant Green : 0.01
Ammonium bismuth citrate : 3.00
Sodium sulfite : 10.00
Disodium hydrogen orthophosphate : 5.00
Beef extract (ex.buffalo) : 6.00
Directions :
1. Add 63.40 gm powder to distilled/deionized water.
2. Bring volume to 1.0 litre and mix thoroughly.
3. Gently heat and bring to boiling.
4. Do not Autoclave or Overheat the medium.
5. Swirl the flask while dispensing into sterile petri plates.
More Information
Product Code 55369-100 Gms
HSN Code 38210000
Brand SRL
Usually dispatched in 7-10 DAYS
Pack Size 100 Gms
Purity % Salmonella typhimurium ATCC 14028, Salmonella typhi ATCC 6539, Escherichia coli ATCC 25922, Enterococcus faecalis ATCC 29212, Salmonella enteritidis ATCC 13076

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DESCRIPTION

(Bismuth Sulfite Agar (I))
Concentration 63.40 gm/lit.
Storage : 8 to 25°C (Cool & dry area)
Shelf Life : 36 Months

Specifications
Appearance (Colour) Light beige to light green
Appearance (Form) Free flowing, homogeneous powder
Solubility 63.40 gm/liter
Solubility before autoclaving (Clarity) Opaque with a flocculent precipitate
Gel strength Firm, comparable with 1.5% agar gel
pH (25°C) 7.5 ± 0.2
Prepared medium appearance after boiling (clarity) Opaque with a flocculent precipitate
Prepared medium appearance after boiling (colour) Light grey-green to medium green
Cultural Response Inoculate and incubate at 37°C for 18 -24 hours
Organism Salmonella typhimurium ATCC 14028
Inoculum (cfu) 10-100 Growth : Good, Recovery Rate : >=50%, Colony colour : Black or greenish-grey, may have sheen
Organism Salmonella typhi ATCC 6539
Inoculum (cfu) 10-100 Growth : Good, Recovery Rate : >=50%, Colony colour : Black with metallic sheen
Organism Escherichia coli ATCC 25922
Inoculum >103, Growth : Partial Inhibition, Colony colour : Dull green or brown colonies, without metallic sheen.
Organism Enterococcus faecalis ATCC 29212
Inoculum >103, Growth : Inhibited
Organism Salmonella enteritidis ATCC 13076
Inoculum (cfu) 10-100 Growth : Good, Recovery Rate : >=50%, Colony colour : Black with metallic sheen
Notes
  Applications :
For the selective isolation and identification of Salmonella typhi and other enteric bacilli.
Composition:
Ingredients : gm/lt.
Peptone : 10.00
Agar : 24.00
D- Glucose monohydrate : 5.00
Ferric citrate : 0.40
Brilliant Green : 0.01
Ammonium bismuth citrate : 3.00
Sodium sulfite : 10.00
Disodium hydrogen orthophosphate : 5.00
Beef extract (ex.buffalo) : 6.00
Directions :
1. Add 63.40 gm powder to distilled/deionized water.
2. Bring volume to 1.0 litre and mix thoroughly.
3. Gently heat and bring to boiling.
4. Do not Autoclave or Overheat the medium.
5. Swirl the flask while dispensing into sterile petri plates.
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