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BglII (10U/mcl)

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₹2,200.00 + GST 5% off
₹2,090.00 + ₹250.80 GST
₹2,340.80 (Incl. of all taxes)
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Overview
Specifications
Appearance (Form) Liquid
Appearance (Colour) Colourless
Appearance (Clarity) Clear
Activity One unit is defined as the amount of enzyme required to digest 1 μg of lambda DNA in 1 hour at 37 °C in 50 μL of assay buffer.
DNA degradation Incubation of single stranded and double stranded radiolabeled oligonucleotides with 10 units of the enzyme for 4 hours at 37 °C.
Star Acitvity After 160-fold overdigestion (10 U/μg Lambda DNA for 16 hours at 37 °C) with enzyme the fragmentation pattern is analysed. No detectable changes compared to the theoretical fragmentation pattern are considered as absence of star activity.
Notes
  Description:
BglII is an endonuclease enzyme isolated from strains of Bacillus globigii lacking BglII and is part of the restriction modification system. In molecular biology it is used as a restriction enzyme that creates sticky ends. The nucleic acid sequence where the enzyme cuts is AGATCT, which as the complementary sequence is TCTAGA.

Includes:
250U
• BglII - 25 μl
• 10X Reaction Buffer - 1.0ml
More Information
Product Code 24848-250 Units
HSN Code 38229090
Brand SRL
Usually dispatched in 2-3WEEK
Pack Size 250 Units
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  1. BglII (10U/mcl)
    BglII (10U/mcl)
    ₹2,200.00 + GST 5% off
    ₹2,090.00 + ₹250.80 GST
    ₹2,340.80 (Incl. of all taxes)
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Details
Specifications
Appearance (Form) Liquid
Appearance (Colour) Colourless
Appearance (Clarity) Clear
Activity One unit is defined as the amount of enzyme required to digest 1 μg of lambda DNA in 1 hour at 37 °C in 50 μL of assay buffer.
DNA degradation Incubation of single stranded and double stranded radiolabeled oligonucleotides with 10 units of the enzyme for 4 hours at 37 °C.
Star Acitvity After 160-fold overdigestion (10 U/μg Lambda DNA for 16 hours at 37 °C) with enzyme the fragmentation pattern is analysed. No detectable changes compared to the theoretical fragmentation pattern are considered as absence of star activity.
Notes
  Description:
BglII is an endonuclease enzyme isolated from strains of Bacillus globigii lacking BglII and is part of the restriction modification system. In molecular biology it is used as a restriction enzyme that creates sticky ends. The nucleic acid sequence where the enzyme cuts is AGATCT, which as the complementary sequence is TCTAGA.

Includes:
250U
• BglII - 25 μl
• 10X Reaction Buffer - 1.0ml
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