B12 Inoculum Broth, (U/P)

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₹7,646.40 (Incl. of all taxes)

In stock

Overview

DESCRIPTION

Concentration 32.10 gm/lit.
Storage : 2 to 8°C (Refrigerate)
Shelf Life : 36 Months

Specifications

Composition :
Peptone : 7.50 gm/lit.
Yeast extract : 7.50 gm/lit.
Dextrose :10.00 gm/lit.
Monopotassium
phosphate :2.00 gm/lit.
Tomato juice : 5.00 gm/lit.
Polysorbate 80 : 0.10 gm/lit.
pH at 25 °C : 6.8 ± 0.2

Notes

Directions :
1.Add 32.1 gm powder to distilled/deionized water.
2.Bring volume to 1.0 liter and mix thoroughly.
3.Heat gently and bring to boiling.
4.Distribute into tubes in 10 ml volumes.
5.Autoclave at 15 psi pressure at 121°C for 15 minutes.
6.Cool tubes in an upright position.
Applications:
For the preparation of inoculum cultures of Lactobacillus leichmannii ATCC 7830, which is used as the test organism in the vitamin B12 assay according to the USP.