B12 Culture Agar, (U/P)

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~~₹1,900.00 + GST~~ 8% off
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₹2,062.64 (Incl. of all taxes)

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Overview

DESCRIPTION

Concentration 42.10 gm/lit.
Storage : 2 to 8°C (Refrigerate)
Shelf Life : 36 Months

Specifications

Composition :
Peptone : 7.50 gm/lit.
Yeast extract : 7.50 gm/lit.
Dextrose : 10.00 gm/lit.
Monopotassium
phosphate : 2.00 gm/lit.
Tomato juice : 5.00 gm/lit.
Polysorbate 80 : 0.10 gm/lit.
Agar : 10.0 gm/lit.
pH at 25 °C : 6.8 ± 0.2

Notes

Applications:
For the cultivation and maintenance of Lactobacillus leichmannii ATCC 7830 to be used as the test organism in the vitamin B12 assay according to the USP.
Directions :
1.Add 42.1 gm powder to distilled/deionized water.
2.Bring volume to 1.0 liter and mix thoroughly.
3.Heat gently and bring to boiling.
4.Distribute into tubes in 10 ml volumes.
5.Autoclave at 15 psi pressure at 121°C for 15 minutes.
6.Cool tubes in an upright position.