B.C.P. - D.C.L.S. Agar

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Overview

DESCRIPTION

(Bromocresol Purple-Deoxycholic Acid-Citrate-Lactose-Sucrose Agar)
Concentration 67.52 gm/lit.
Storage : 8 to 25°C (Cool & dry area)
Shelf Life : 36 Months

Specifications
Appearance (Colour) Beige
Appearance (Form) Free flowing, homogeneous powder
Solubility 67.52 gm/liter
Solubility before boiling (clarity) Clear to slightly opalescent
Gel strength (1.5% gel) Firm, comparable with 1.5% agar gel
pH (25°C) 7.2 ± 0.2
Prepared medium appearance after boiling (clarity) Clear to slightly opalescent
Prepared medium appearance after boiling (colour) Purple coloured
Cultural Response Inoculate and incubate at 35-37°C for 18-24 hours
Organism Escherichia coli ATCC 25922
Inoculum (cfu) 10-100 Growth : None-poor
Recovery rate : <=10.0%
Colour of colony : yellow
Organism Proteus mirabilis ATCC 12453
Inoculum (cfu) 10-100 Growth : None-poor
Recovery rate : <=10.0%
Colour of colony : colourless
Organism Salmonella enteritidis ATCC 13076
Inoculum (cfu) 10-100 Growth : Good
Recovery rate : <=50.0%
Colour of colony : colourless may show faint bluish coloured colonies
Organism Shigella dysenteriae ATCC 13313
Inoculum (cfu) 10-100 Growth : Good
Recovery rate : <=50.0%
Colour of colony : colourless may show faint bluish coloured colonies
Organism Shigella flexneri ATCC 12022
Inoculum (cfu) 10-100 Growth : Good
Recovery rate : <=50.0%
Colour of colony : colourless may show faint bluish coloured colonies
Organism Salmonella typhimurium ATCC 14028
Inoculum (cfu) 10-100 Growth : Good
Recovery rate : <=50.0%
Colour of colony : colourless may show faint bluish coloured colonies
Organism Shigella sonnei ATCC 25931
Inoculum (cfu) 10-100 Growth : Good
Recovery rate : <=50.0%
Colour of colony : colourless may show faint bluish coloured colonies
Notes
  Applications:
Used for the selective isolation of Salmonella and Shigella species.
Composition:
Ingredients : gm/lt.
Peptic digest of animal tissue : 5.00
Yeast extract : 3.00
Beef extract(ex.buffalo) : 3.00
Pancreatic digest of casein : 5.00
Lactose : 7.50
Sucrose : 7.50
Sodium citrate : 10.00
Sodium chloride : 5.00
Sodium thiosulphate : 5.00
Sodium deoxycholate : 2.50
Bromocresol Purple : 0.02
Agar : 14.00
Directions:
1. Add 67.52 gm powder to 1.0 litre distilled/deionized water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Cool to 45- 50°C.
4. Do not autoclave.
More Information
Product Code 97676-500 Gms
HSN Code 38210000
Brand SRL
Usually dispatched in 7-10 DAYS
Pack Size 500 Gms
Purity % Escherichia coli ATCC 25922, Proteus mirabilis ATCC 12453, Salmonella enteritidis ATCC 13076, Shigella dysenteriae ATCC 13313, Shigella flexneri ATCC 12022, Salmonella typhimurium ATCC 14028, Shigella sonnei ATCC 25931

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DESCRIPTION

(Bromocresol Purple-Deoxycholic Acid-Citrate-Lactose-Sucrose Agar)
Concentration 67.52 gm/lit.
Storage : 8 to 25°C (Cool & dry area)
Shelf Life : 36 Months

Specifications
Appearance (Colour) Beige
Appearance (Form) Free flowing, homogeneous powder
Solubility 67.52 gm/liter
Solubility before boiling (clarity) Clear to slightly opalescent
Gel strength (1.5% gel) Firm, comparable with 1.5% agar gel
pH (25°C) 7.2 ± 0.2
Prepared medium appearance after boiling (clarity) Clear to slightly opalescent
Prepared medium appearance after boiling (colour) Purple coloured
Cultural Response Inoculate and incubate at 35-37°C for 18-24 hours
Organism Escherichia coli ATCC 25922
Inoculum (cfu) 10-100 Growth : None-poor
Recovery rate : <=10.0%
Colour of colony : yellow
Organism Proteus mirabilis ATCC 12453
Inoculum (cfu) 10-100 Growth : None-poor
Recovery rate : <=10.0%
Colour of colony : colourless
Organism Salmonella enteritidis ATCC 13076
Inoculum (cfu) 10-100 Growth : Good
Recovery rate : <=50.0%
Colour of colony : colourless may show faint bluish coloured colonies
Organism Shigella dysenteriae ATCC 13313
Inoculum (cfu) 10-100 Growth : Good
Recovery rate : <=50.0%
Colour of colony : colourless may show faint bluish coloured colonies
Organism Shigella flexneri ATCC 12022
Inoculum (cfu) 10-100 Growth : Good
Recovery rate : <=50.0%
Colour of colony : colourless may show faint bluish coloured colonies
Organism Salmonella typhimurium ATCC 14028
Inoculum (cfu) 10-100 Growth : Good
Recovery rate : <=50.0%
Colour of colony : colourless may show faint bluish coloured colonies
Organism Shigella sonnei ATCC 25931
Inoculum (cfu) 10-100 Growth : Good
Recovery rate : <=50.0%
Colour of colony : colourless may show faint bluish coloured colonies
Notes
  Applications:
Used for the selective isolation of Salmonella and Shigella species.
Composition:
Ingredients : gm/lt.
Peptic digest of animal tissue : 5.00
Yeast extract : 3.00
Beef extract(ex.buffalo) : 3.00
Pancreatic digest of casein : 5.00
Lactose : 7.50
Sucrose : 7.50
Sodium citrate : 10.00
Sodium chloride : 5.00
Sodium thiosulphate : 5.00
Sodium deoxycholate : 2.50
Bromocresol Purple : 0.02
Agar : 14.00
Directions:
1. Add 67.52 gm powder to 1.0 litre distilled/deionized water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Cool to 45- 50°C.
4. Do not autoclave.
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