Alternative Thioglycollate Medium (I/P)(U/P)

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Overview

Description:

(NIH Thioglycollate Broth)

Concentration 29 gm/lit.
Storage : 8 to 25°C (Cool & dry area)
Shelf Life : 36 Months

Specifications
Appearance (Colour) Beige to tan
Appearance (Form) Free flowing, homogeneous powder
Solubility 29.00 gm/liter
Solubility before autoclaving (Clarity) Clear to very slightly opalescent may have slight precipitate
pH (25°C) 7.1 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity) Clear to very slightly opalescent may have slight precipitate
Prepared Medium Appearance after autoclaving (Colour) Light amber
Cultural Response Inoculate and incubate at 30-35°C for upto 24 - 72 hours
Organism Staphylococcus aureus ATCC 25923
Inoculum (cfu) 10-100 Growth : Good
Organism Clostridium sporogenes ATCC 11437
Inoculum (cfu) 10-100 Growth : Good
Organism Bacillus subtilis ATCC 6633
Inoculum (cfu) 10-100 Growth : Good
Organism Bacteroides vulgatus ATCC 8482
Inoculum (cfu) 10-100 Growth : Good
Organism Candida albicans ATCC 10231
Inoculum (cfu) 10-100 Growth : Good
Organism Escherichia coli ATCC 25922
Inoculum (cfu) 10-100 Growth : Good
Organism Streptococcus pyogenes ATCC 19615
Inoculum (cfu) 10-100 Growth : Good
 
Notes
  Applications:
For the sterility testing of biological products which are turbid or viscous as mentioned in pharmacopeia.

Composition:
Ingredients : gm/lt.
Yeast extract : 5.00
Enzymatic hydrolysate of casein : 15.00
Dextrose : 5.50
Sodium chloride : 2.50
L-Cystine : 0.50
Sodium thioglycollate : 0.50

Directions:

1. Add 29.00 gm powder to 1.0 liter distilled/deionized water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Dispense into test tubes.
4. Autoclave at 15 psi pressure at 121°C for 15 minutes.
More Information
Product Code 52023-100 Gms
HSN Code 38210000
Brand SRL
Usually dispatched in 7-10 DAYS
Pack Size 100 Gms
Purity % Staphylococcus aureus ATCC 25923, Clostridium sporogenes ATCC 11437, Bacillus subtilis ATCC 6633, Bacteroides vulgatus ATCC 8482, Candida albicans ATCC 10231, Escherichia coli ATCC 25922, Streptococcus pyogenes ATCC 19615

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Description:

(NIH Thioglycollate Broth)

Concentration 29 gm/lit.
Storage : 8 to 25°C (Cool & dry area)
Shelf Life : 36 Months

Specifications
Appearance (Colour) Beige to tan
Appearance (Form) Free flowing, homogeneous powder
Solubility 29.00 gm/liter
Solubility before autoclaving (Clarity) Clear to very slightly opalescent may have slight precipitate
pH (25°C) 7.1 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity) Clear to very slightly opalescent may have slight precipitate
Prepared Medium Appearance after autoclaving (Colour) Light amber
Cultural Response Inoculate and incubate at 30-35°C for upto 24 - 72 hours
Organism Staphylococcus aureus ATCC 25923
Inoculum (cfu) 10-100 Growth : Good
Organism Clostridium sporogenes ATCC 11437
Inoculum (cfu) 10-100 Growth : Good
Organism Bacillus subtilis ATCC 6633
Inoculum (cfu) 10-100 Growth : Good
Organism Bacteroides vulgatus ATCC 8482
Inoculum (cfu) 10-100 Growth : Good
Organism Candida albicans ATCC 10231
Inoculum (cfu) 10-100 Growth : Good
Organism Escherichia coli ATCC 25922
Inoculum (cfu) 10-100 Growth : Good
Organism Streptococcus pyogenes ATCC 19615
Inoculum (cfu) 10-100 Growth : Good
 
Notes
  Applications:
For the sterility testing of biological products which are turbid or viscous as mentioned in pharmacopeia.

Composition:
Ingredients : gm/lt.
Yeast extract : 5.00
Enzymatic hydrolysate of casein : 15.00
Dextrose : 5.50
Sodium chloride : 2.50
L-Cystine : 0.50
Sodium thioglycollate : 0.50

Directions:

1. Add 29.00 gm powder to 1.0 liter distilled/deionized water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Dispense into test tubes.
4. Autoclave at 15 psi pressure at 121°C for 15 minutes.
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