Alicyclobacillus Detection Agar (Alicyclobacillus Agar)

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₹2,442.60 (Incl. of all taxes)
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Overview

DESCRIPTION

Concentration 29.00 gm/lit.

Storage : 8 to 25°C (Cool & dry area)
Shelf Life : 36 Months

Specifications
Appearance (Colour) Beige to light beige
Appearance (Form) Free flowing, homogeneous powder
Solubility 29.00 gm/liter
Solubility before autoclaving (Clarity) Clear to slightly opalescent
Gel strength Firm comparable with 1.5% agar gel
pH (25°C) 4.0 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity) Clear to slightly opalescent
Prepared Medium Appearance after autoclaving (Colour) Light amber
Cultural Response Inoculate and incubate at 45°C for 18-24 hours.
Organism Alicyclobacillus acidocaldarius ATCC 27009
Inoculum (cfu) 10-100 Growth (30°C) : Good
Organism Escherichia coli ATCC 25922
Inoculum (cfu) 10-100 Growth (30°C) : Inhibited
Organism Staphylococcus aureus ATCC 25923
Inoculum (cfu) 10-100 Growth (30°C) : Inhibited
Organism Candida albicans ATCC 10231
Inoculum (cfu) 10-100 Growth (30°C) : Inhibited
Organism Saccharomyces cerevisiae ATCC 9763
Inoculum (cfu) 10-100 Growth (30°C) : Inhibited
Notes
  Applications:
Used for detection of Alicyclobacillus in fruit juices.
Composition:
Ingredients : gm/lt.
Glucose : 5.00
Potassium dihydrogen phosphate : 3.00
Yeast extract : 2.00
Magnesium sulphate : 0.50
Calcium chloride : 0.25
Copper sulphate : 0.00016
Ammonium sulphate : 0.20
Cobalt chloride : 0.00018
Sodium molybdate : 0.00030
Zinc sulfate : 0.00018
Mangenese sulfate : 0.00015
Boric acid : 0.00010
Agar : 18.00
Directions:
1. Add 29.00 gm to 1.0 liter distilled/deionized water and mix throughly.
2. Gently heat and bring to boil to dissolve medium completely.
3. Autoclave at 15 psi at 121°C for 15 minutes.
Note : The media is manufactured with pH 5.3±0.2 to maintain gel strength during sterilization. Adjust the pH at 4.0±0.2 after autoclaving, by adding 1N H2SO4 OR 1N NaOH.
More Information
Product Code 45712-100 Gms
HSN Code 38210000
Brand SRL
Usually dispatched in 7-10 DAYS
Pack Size 100 Gms
Purity % Alicyclobacillus acidocaldarius ATCC 27009, Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 25923, Candida albicans ATCC 10231, Saccharomyces cerevisiae ATCC 9763

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DESCRIPTION

Concentration 29.00 gm/lit.

Storage : 8 to 25°C (Cool & dry area)
Shelf Life : 36 Months

Specifications
Appearance (Colour) Beige to light beige
Appearance (Form) Free flowing, homogeneous powder
Solubility 29.00 gm/liter
Solubility before autoclaving (Clarity) Clear to slightly opalescent
Gel strength Firm comparable with 1.5% agar gel
pH (25°C) 4.0 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity) Clear to slightly opalescent
Prepared Medium Appearance after autoclaving (Colour) Light amber
Cultural Response Inoculate and incubate at 45°C for 18-24 hours.
Organism Alicyclobacillus acidocaldarius ATCC 27009
Inoculum (cfu) 10-100 Growth (30°C) : Good
Organism Escherichia coli ATCC 25922
Inoculum (cfu) 10-100 Growth (30°C) : Inhibited
Organism Staphylococcus aureus ATCC 25923
Inoculum (cfu) 10-100 Growth (30°C) : Inhibited
Organism Candida albicans ATCC 10231
Inoculum (cfu) 10-100 Growth (30°C) : Inhibited
Organism Saccharomyces cerevisiae ATCC 9763
Inoculum (cfu) 10-100 Growth (30°C) : Inhibited
Notes
  Applications:
Used for detection of Alicyclobacillus in fruit juices.
Composition:
Ingredients : gm/lt.
Glucose : 5.00
Potassium dihydrogen phosphate : 3.00
Yeast extract : 2.00
Magnesium sulphate : 0.50
Calcium chloride : 0.25
Copper sulphate : 0.00016
Ammonium sulphate : 0.20
Cobalt chloride : 0.00018
Sodium molybdate : 0.00030
Zinc sulfate : 0.00018
Mangenese sulfate : 0.00015
Boric acid : 0.00010
Agar : 18.00
Directions:
1. Add 29.00 gm to 1.0 liter distilled/deionized water and mix throughly.
2. Gently heat and bring to boil to dissolve medium completely.
3. Autoclave at 15 psi at 121°C for 15 minutes.
Note : The media is manufactured with pH 5.3±0.2 to maintain gel strength during sterilization. Adjust the pH at 4.0±0.2 after autoclaving, by adding 1N H2SO4 OR 1N NaOH.
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