5' HEX (1 OD)
₹18,290.00 (Incl. of all taxes)
| Product Code | 103100011730 - BGC-5HEXA |
|---|---|
| HSN Code | 998349 |
| Brand | Bangalore Genei |
| Usually dispatched in | On request |
| Pack Size | Per Modification |
DESCRIPTION:
Genei offers custom oligonucleotide service to scientists nationwide.
Features:
- Oligonucleotides available as (a) Crude (b) Desalted (c) PAGE purified.
- Choice of purification (a) Desalting: using sephadex G-25 columns (b) PAGE purification: 20% polyacrylamide + 7M urea gel electrophoresis.
- Modifications available (a) Inosine – at any position other than 3′ end (b) Biotin and Fluorescein – only at 5′ end.
- Certificate of analysis includes primer length, sequence, yield, concentration and purity.
- Product quality checked by PAGE analysis of the primer.
- Oligos will be supplied in lyophilized form.
- Quick delivery.
Note:
- Order should accompany primer sequence with indication of 5′ – 3′ ends.
- No mixed bases at 3′ end.
- Please use the IUPAC-code for mixed bases
(M=A/C, R=A/G, W=A/T, Y=C/T, S=C/G, K=G/T, H=A/C/T, V=A/C/G, D=A/G/T, B=C/G/T, N=A/C/G/T).
Crude oligo: Deprotected oligo with 85-90% of full length product along with 10-15% of failure sequences, salts and protective chemical groups.
Application: Standard PCR, Hybridization, Probing etc.
Desalted oligo: Desalting of crude oligo on Sephadex G-25 column, removes ammonium salts and protective chemical groups which gives 85 – 90% of full length product along with 10-15% of failure.
Application: PCR, Probing, Cycle sequencing, Hybridization, Primer extension.
PAGE Purified oligo: Crude oligo purified on 20% Urea PAGE followed by desalting on Sephadex G-25 yields 95-99% purity. Total yield is lower due to relative ineffective recovery of oligo from the gel and Sephadex G-25 column. The basis of separation of the oligo is by molecular weight and charge.
Application: Cloning, RT-PCR, Site Directed Mutagenesis, Gel Shift Assay, DNA fingerprinting, in situ hybridization, Microsatellite polymorphism, Gene synthesis.